• Product nameAnti-Calretinin antibody
    See all Calretinin primary antibodies
  • Description
    Rabbit polyclonal to Calretinin
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cat, Dog, Human, Xenopus laevis, Zebrafish
  • Immunogen

    Full length protein

  • Positive control
    • Mesothelioma.
  • General notesContains a proprietary preservative that is not sodium azide or thimerosal.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, Carrier protein, Van Gogh Yellow Diluent, pH 7.3
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • Myelomaunknown
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.
IHC-Fr 1/100. PubMed: 20504962

Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).


Anti-Calretinin antibody images

  • ab702 staining Calretinin in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab702 staining Calretinin (green) in zebrafish retinal cells by Immunohistochemistry (frozen sections).
    Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat.

    Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped.

    Original article: http://dev.biologists.org/content/137/13/2107.long
  • Immunohistochemical analysis of sea cucumber radial nerve cord tissue, staining Calretinin with ab702 at 1/100 dilution.

References for Anti-Calretinin antibody (ab702)

This product has been referenced in:
  • Taylor SR  et al. Neuroblast Distribution after Cortical Impact Is Influenced by White Matter Injury in the Immature Gyrencephalic Brain. Front Neurosci 10:387 (2016). Read more (PubMed: 27601978) »
  • Fatt MP  et al. p63 and p73 coordinate p53 function to determine the balance between survival, cell death, and senescence in adult neural precursor cells. Cell Death Differ N/A:N/A (2014). Read more (PubMed: 24809925) »

See all 15 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample Mouse Tissue sections (frozen brain section)
Specification frozen brain section
Permeabilization No
Fixative Paraformaldehyde

Mrs. Bettina Hoerz

Verified customer

Submitted Jan 06 2014

We do not have specific use by dates for our products.

If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

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Gracias por tu respuesta.

Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas ...

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Gracias por contactarnos.

Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuaci...

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