Overview

  • Product name
  • Description
    Rabbit polyclonal to Calretinin
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Chicken, Cat, Dog, Xenopus laevis
  • Immunogen

    Full length protein corresponding to Calretinin.

  • Positive control
    • IHC-P: Mesothelioma. IHC-Fr: Mouse brain, zebrafish retinal tissue and sea cucumber radial nerve cord tissue.
  • General notes
    Contains a proprietary preservative that is not sodium azide or thimerosal.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.09% Sodium azide
    Constituents: PBS, Carrier protein

    Van Gogh Yellow Diluent
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Myeloma
    unknown
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.

IHC-Fr 1/100. PubMed: 20504962

Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).

Target

Anti-Calretinin antibody images

  • Paraffin embedded mesothelioma stained for Calretinin with ab702 (1/100 dilution) in immunohistochemical analysis.

  • ab702 staining Calretinin in mouse brain tissue sections in immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab702 staining Calretinin (green) in zebrafish retinal cells by immunohistochemistry (frozen sections). Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat. Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped. Original article: http://dev.biologists.org/content/137/13/2107.long

  • Immunohistochemical analysis of sea cucumber radial nerve cord tissue, staining Calretinin with ab702 at 1/100 dilution.

References for Anti-Calretinin antibody (ab702)

This product has been referenced in:
  • Taylor SR  et al. Neuroblast Distribution after Cortical Impact Is Influenced by White Matter Injury in the Immature Gyrencephalic Brain. Front Neurosci 10:387 (2016). IHC-FoFr ; Pig . Read more (PubMed: 27601978) »
  • Morrone CD  et al. Effects of Neurotrophic Support and Amyloid-Targeted Combined Therapy on Adult Hippocampal Neurogenesis in a Transgenic Model of Alzheimer's Disease. PLoS One 11:e0165393 (2016). ICC/IF ; Mouse . Read more (PubMed: 27768761) »

See all 21 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Fukomys anselli Tissue sections (Retina)
Permeabilization
Yes - Triton X-100 in Blocking Solution
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Davidson's Fixative
Username

Abcam user community

Verified customer

Submitted Jul 31 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample
Mouse Tissue sections (frozen brain section)
Specification
frozen brain section
Permeabilization
No
Fixative
Paraformaldehyde
Username

Mrs. Bettina Hoerz

Verified customer

Submitted Jan 06 2014



We do not have specific use by dates for our products.

If stored correctly, antibodies remain suitable for use for months and even for years. We are pleased to provide our guarantee for 1 year after purchase.

Please review...

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Gracias por tu respuesta.

Siento comunicarte que todas las publicaciones que tenemos disponibles sobre el anticuerpo ab702 son las presentes en la datasheet.

Tenemos otros anticuerpos contra Calretinina, quizás en sus fichas ...

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Gracias por contactarnos.


Algunos de estos artículos son de libre acceso, y pueden consultarse online, este es el caso de los artículos citados en primer, tercer, sexto, séptimo y octavo lugar. Los links a continuaci...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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