Calretinin antibody (ab702)

Product Name 

Calretinin antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Calretinin

Immunogen 

Full length protein

Reacts with 
(species key)

Hu, Ms, Rat, Cat, Chk, Xl

Tested applications 
(see key)

IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC:
For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT.
For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before performing IHC.
Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas.
However, it is recommended that a panel of antibodies be used in tandem with calretinin.
Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15.
Calretinin does not mark all mesotheliomas (50-70%).

Positive control 
(see definition)

Mesothelioma.

Cellular localization 

Cytoplasmic

Research areas 

Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins

Relevance 

Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. The antibodies against calretinin also labels the mossy fibers throughout, and climbing fibers in a distinct group of folia. They also stain several cell types in the molecular layer. There is no appreciable immunostaining of calretinin in cerebellar cortex. In the rat, antibodies against calretinin stain the Lugaro cells, and some granule cells in lobe X; they also give weak staining of all the granule cells in the other lobes. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Van Gogh Yellow Diluent, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C.

Notes 

Contains a proprietary preservative that is not sodium azide or thimerosal.

References for Calretinin antibody (ab702)

  This product has been used in: (two most recent references)

Pinaud R et al. Profiling of experience-regulated proteins in the songbird auditory forebrain using quantitative proteomics. Eur J Neurosci 27:1409-22 (2008). PubMed: 18364021

Harrison SJ et al. Sall1 Regulates Mitral Cell Development and Olfactory Nerve Extension in the Developing Olfactory Bulb. Cereb Cortex : (2007). PubMed: 18024993

See all 10 publication references for this product.


If you publish research using ab702 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Calretinin antibody

Customer FAQs regarding Calretinin antibody

Protocols for Calretinin antibody

Price and availability of products related to Calretinin antibody

Calretinin antibody - related products: