Anti-Calretinin antibody [EP1798] (ab92341)

Overview

  • Product nameAnti-Calretinin antibody [EP1798]
    See all Calretinin primary antibodies
  • Description
    Rabbit monoclonal [EP1798] to Calretinin
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Calretinin aa 50-150. The exact sequence is proprietary.
    Database link: P22676

  • Positive control
    • Human fetal, Mouse and Rat brain lysates; Human brain and mesothelioma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP1798
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
Flow Cyt
  • Application notesFlow Cyt: 1/100.
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    WB: 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Calretinin antibody [EP1798] images

    • ab92341 at 1/100 dilution staining Calretinin in Human mesothelioma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
    • ab92341 at 1/100 dilution staining Calretinin in Human brain by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
    • All lanes : Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution

      Lane 1 : Human fetal Brain lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Overlay histogram showing SH-SY5Y cells stained with ab92341 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92341, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-Calretinin antibody [EP1798] (ab92341)

    This product has been referenced in:
    • Francavilla C  et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017). ICC/IF ; Human . Read more (PubMed: 28355574) »
    • McMahon SM  et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016). WB ; Rat . Read more (PubMed: 26880753) »

    See all 5 Publications for this product

    Product Wall

    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (brainstem)
    Specification brainstem
    Username

    Lin Lin

    Verified customer

    Submitted Jun 16 2017

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Permeabilization Yes - 0.1% Triton-X (in PBS)
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 12 2013


    The SH-SY5Y cell line is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH. SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"