Recombinant
RabMAb

Anti-Calretinin antibody [EP1798] (ab92341)

Overview

  • Product name
    Anti-Calretinin antibody [EP1798]
    See all Calretinin primary antibodies
  • Description
    Rabbit monoclonal [EP1798] to Calretinin
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Calretinin aa 50-150. The exact sequence is proprietary.
    Database link: P22676

  • Positive control
    • Human fetal, Mouse and Rat brain lysates; Human brain and mesothelioma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP1798
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
Flow Cyt
  • Application notes
    Flow Cyt: 1/100.
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.
    WB: 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • ab92341 at 1/100 dilution staining Calretinin in Human mesothelioma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
    • ab92341 at 1/100 dilution staining Calretinin in Human brain by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
    • All lanes : Anti-Calretinin antibody [EP1798] (ab92341) at 1/1000 dilution

      Lane 1 : Human fetal Brain lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • Overlay histogram showing SH-SY5Y cells stained with ab92341 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92341, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References

    This product has been referenced in:
    • Francavilla C  et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017). ICC/IF ; Human . Read more (PubMed: 28355574) »
    • McMahon SM  et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016). WB ; Rat . Read more (PubMed: 26880753) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brainstem)
    Specification
    brainstem
    Username

    Lin Lin

    Verified customer

    Submitted Jun 16 2017

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.1% Triton-X (in PBS)
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 12 2013


    The SH-SY5Y cell line is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH. SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up