Anti-Calsequestrin 2 antibody (ab96387)
Key features and details
- Rabbit polyclonal to Calsequestrin 2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Calsequestrin 2 antibody
See all Calsequestrin 2 primary antibodies -
Description
Rabbit polyclonal to Calsequestrin 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment corresponding to Human Calsequestrin 2 aa 124-398.
Database link: O14958 -
Positive control
- IHC-P: Cal27 xenograft, mouse muscle tissue. WB: H1299 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. |
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
Target
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Function
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. -
Involvement in disease
Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) [MIM:611938]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. -
Sequence similarities
Belongs to the calsequestrin family. -
Cellular localization
Sarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells. - Information by UniProt
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Database links
- Entrez Gene: 845 Human
- Entrez Gene: 12373 Mouse
- Entrez Gene: 29209 Rat
- Omim: 114251 Human
- SwissProt: O14958 Human
- SwissProt: O09161 Mouse
- SwissProt: P51868 Rat
- Unigene: 57975 Human
see all -
Alternative names
- AA033488 antibody
- AW146219 antibody
- Calsequestrin 2 (cardiac muscle) antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96387 at 1:500 dilution.
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Anti-Calsequestrin 2 antibody (ab96387) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE -
Paraffin embedded mouse muscle tissue stained for Calsequestrin-2 using ab96387 at 1/500 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab96387 has not yet been referenced specifically in any publications.