Anti-Calsequestrin antibody (ab97474)

Overview

  • Product nameAnti-Calsequestrin antibody
    See all Calsequestrin primary antibodies
  • Description
    Rabbit polyclonal to Calsequestrin
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 42-271 of Human Calsequestrin (NP_001222)

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab97474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-Calsequestrin antibody images

  • IHC image of Calsequestrin staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Calsequestrin antibody (ab97474) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 44 kDa
    10% SDS PAGE

References for Anti-Calsequestrin antibody (ab97474)

ab97474 has not yet been referenced specifically in any publications.

Product Wall

I would recommened ab3516, it has been tested in more applications and species.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (rear leg muscle)
Loading amount 10 µg
Specification rear leg muscle
Gel Running Conditions Reduced Denaturing (10-20% tricine)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - other (SR Prep)
Loading amount 10 µg
Specification SR Prep
Gel Running Conditions Reduced Denaturing (10-20% Tricine)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"