Overview

  • Product name
  • Description
    Rabbit polyclonal to Calumenin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Calumenin.

  • Positive control
    • This antibody gave a positive signal in the following Human tissue lysates: Placenta; Skeletal muscle; Lung.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 37 kDa).

Target

  • Function
    Involved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
  • Tissue specificity
    Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver.
  • Sequence similarities
    Belongs to the CREC family.
    Contains 6 EF-hand domains.
  • Cellular localization
    Endoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CALU antibody
    • CALU_HUMAN antibody
    • Calumenin 1 antibody
    • Calumenin 2 antibody
    • Calumenin antibody
    • Crocalbin antibody
    • crocalbin-like protein antibody
    • FLJ90608 antibody
    • IEF SSP 9302 antibody
    • multiple EF-hand protein antibody
    • OTTHUMP00000212712 antibody
    • OTTHUMP00000212713 antibody
    • OTTHUMP00000212714 antibody
    • OTTHUMP00000212715 antibody
    see all

Images

  • All lanes : Anti-Calumenin antibody (ab102115) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330) with Immunising peptide at 1 µg/ml
    Lane 6 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa,30 kDa,42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References

ab102115 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab102115.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up