Anti-CaMK2 beta gamma delta antibody (ab79359)
Key features and details
- Rabbit polyclonal to CaMK2 beta gamma delta
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CaMK2 beta gamma delta antibody
See all CaMK2 beta gamma delta primary antibodies -
Description
Rabbit polyclonal to CaMK2 beta gamma delta -
Host species
Rabbit -
Specificity
Antibody detects endogenous levels of total CaMK2-beta/gamma/delta protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthesized non-phosphopeptide derived from human CaMK2-beta/gamma/delta around the phosphorylation site of threonine 287 (Q-E-TP-V-E).
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Positive control
- Human brain tissue and rat brain cell extract
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Relevance
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
- Entrez Gene: 171140 Rat
- Entrez Gene: 24245 Rat
see all -
Alternative names
- Calcium/calmodulin-dependent protein antibody
- CaM kinase II beta subunit antibody
- CaM-kinase II beta chain antibody
see all
Images
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using CaMK2-ß/?/d (Ab-287) antibody at 1/50-1/100 dilution.
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All lanes : Anti-CaMK2 beta gamma delta antibody (ab79359) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
Observed band size: ~73 kDa why is the actual band size different from the predicted?
Additional bands at: ~50 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab79359 has not yet been referenced specifically in any publications.