Overview

  • Product nameAnti-CAMK2A antibody
  • Description
    Rabbit polyclonal to CAMK2A
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Horse, Chicken, Cow, Dog, Human, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CAMK2A aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab115931)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Rat brain; Mouse cortex; Rat cortex. IHC-P: FFPE rat brain tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab103840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Anti-CAMK2A antibody images

  • IHC image of CAMK2A staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103840, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-CAMK2A antibody (ab103840) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse cortex Mouse Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 50,54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References for Anti-CAMK2A antibody (ab103840)

ab103840 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"