Overview

  • Product nameAnti-CAMK2G + CAMK2B antibody
  • Description
    Rabbit polyclonal to CAMK2G + CAMK2B
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the C-terminal region of human CAMK2G + CAMK2B.

  • Positive control
    • Extracts from Jurkat cells and COLO205 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 73 kDa).
ELISA 1/20000.

Target

  • RelevanceThe multifunctional CAMK2, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CAMK2 is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory. CAMK2B is the beta chain. There are seven named isoforms produced by alternative splicing. The variable region of the CAMK2B protein is encoded by at least 7 exons (V1 to V7). Alternative splicing within this region gives rise to CAMK2B isoforms. CAMK2G is the gamma chain. CAMK2G has six alternatively spliced variants that encode six different isoforms. Some of these variants have been identified in human tumors.
  • Database links
  • Alternative names
    • CaM kinase II antibody
    • CaM kinase II beta chain antibody
    • CaM kinase II gamma chain antibody
    • CAM2 antibody
    • CAMK antibody
    • CAMK II antibody
    • CaMK II beta subunit antibody
    • CaMK II subunit gamma antibody
    • CAMK2 antibody
    • CAMKB antibody
    • CAMKG antibody
    • Proline rich calmodulin dependent protein kinase antibody
    see all

Anti-CAMK2G + CAMK2B antibody images

  • All lanes : Anti-CAMK2G + CAMK2B antibody (ab72604) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COLO205 cell extract
    Lane 3 : COLO205 cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

References for Anti-CAMK2G + CAMK2B antibody (ab72604)

ab72604 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"