Overview

  • Product name
    Anti-CaMKI antibody [EP2218Y]
    See all CaMKI primary antibodies
  • Description
    Rabbit monoclonal [EP2218Y] to CaMKI
  • Tested applications
    Suitable for: WB, IP, ICC, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminus of human CaMK1

  • Positive control
    • SH-SY5Y cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab62374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP 1/20.
ICC 1/100 - 1/250.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
    • Tissue specificity
      Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Domain
      The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
    • Post-translational
      modifications
      Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
      Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
    • Cellular localization
      Cytoplasm. Nucleus. Predominantly cytoplasmic.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium / calmodulin dependent protein kinase 1 antibody
      • Calcium / calmodulin dependent protein kinase I antibody
      • Calcium / calmodulin dependent protein kinase type 1 antibody
      • Calcium/calmodulin dependent protein kinase 1 antibody
      • Calcium/calmodulin dependent protein kinase I antibody
      • Calcium/calmodulin dependent protein kinase type 1 antibody
      • Calcium/calmodulin-dependent protein kinase type 1 antibody
      • CaM K1 antibody
      • CaM KI antibody
      • CaM kinase 1 alpha antibody
      • CAM kinase 1 antibody
      • CaM kinase I alpha antibody
      • CaM kinase I antibody
      • CaM-KI antibody
      • CaMK 1 antibody
      • CAMK I antibody
      • CaMK1 alpha antibody
      • CAMK1 antibody
      • CAMK1 PEN antibody
      • CaMKI alpha antibody
      • CaMKI-alpha antibody
      • KCC1A_HUMAN antibody
      • MGC120317 antibody
      • MGC120318 antibody
      see all

    Images

    • Anti-CaMKI antibody [EP2218Y] (ab62374) at 1/20000 dilution + SH-SY5Y cell lysate at 10 µg

      Secondary
      goat anti-rabbit, HRP labeled, at 1/2000 dilution

      Predicted band size : 41 kDa
      Observed band size : 41 kDa

    References

    This product has been referenced in:
    • Pfisterer SG  et al. Ca2+/calmodulin-dependent kinase (CaMK) signaling via CaMKI and AMP-activated protein kinase contributes to the regulation of WIPI-1 at the onset of autophagy. Mol Pharmacol 80:1066-75 (2011). WB ; Human . Read more (PubMed: 21896713) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huh-7)
    Loading amount
    10000 cells
    Specification
    huh-7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 26 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up