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Rabbit polyclonal to CaMKI gamma
Predicted to work with:
Mouse, Rat, Cow
conjugated to KLH, corresponding to amino acids 434-450 of Human CAMK1G.
NCI-H460 cell lysate, Human hepatocarcinoma tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
The antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
1/50 - 1/100.
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
May be prenylated on Cys-473.
Cytoplasm. Golgi apparatus membrane. Cell membrane.
Information by UniProt
Calcium/calmodulin dependent protein kinase type 1G antibody
Calcium/calmodulin-dependent protein kinase I-gamma antibody
Calcium/calmodulin-dependent protein kinase Ig antibody
Western blot - Anti-CaMKI gamma antibody (ab37972)
Anti-CaMKI gamma antibody (ab37972) at 1/100 dilution + NCI-H460 cell lysate
Secondary HRP-anti-rabbit Predicted band size: 53 kDa Observed band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKI gamma antibody (ab37972)
ab37972, at a 1/50 dilution, staining CAMK1G in Human hepatocarcinoma tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37972 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
has not yet been referenced specifically in any publications.
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