Overview

  • Product name
  • Description
    Rabbit polyclonal to CaMKI gamma
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.

    (Peptide available as ab38755.)

  • Positive control
    • This antibody gave a positive signal against Human recombinant CAMKI gamma kinase and PC12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab38132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
  • Tissue specificity
    Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • Post-translational
    modifications
    May be prenylated on Cys-473.
  • Cellular localization
    Cytoplasm. Golgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium/calmodulin dependent protein kinase type 1G antibody
    • Calcium/calmodulin-dependent protein kinase I-gamma antibody
    • Calcium/calmodulin-dependent protein kinase Ig antibody
    • Calcium/calmodulin-dependent protein kinase type 1G antibody
    • CaM kinase I gamma antibody
    • CaM kinase IG antibody
    • CaM-KI gamma antibody
    • CaMK like CREB kinase III antibody
    • CaMK-like CREB kinase III antibody
    • Camk1g antibody
    • CaMKI gamma antibody
    • CaMKIG antibody
    • CLICK III antibody
    • CLICK3 antibody
    • dJ272L16.1 antibody
    • KCC1G_HUMAN antibody
    • VWS1 antibody
    see all

Images

  • Anti-CaMKI gamma antibody (ab38132) at 1 µg/ml + Human recombinant CAMKI gamma kinase at 1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 79 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab38132 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab38132, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References

ab38132 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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