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Rabbit polyclonal to CaMKI gamma
Predicted to work with:
Mouse, Cow, Human
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.
(Peptide available as
This antibody gave a positive signal against Human recombinant CAMKI gamma kinase and PC12 cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
Use a concentration of 1 µg/ml.
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
May be prenylated on Cys-473.
Cytoplasm. Golgi apparatus membrane. Cell membrane.
Information by UniProt
Calcium/calmodulin dependent protein kinase type 1G antibody
Calcium/calmodulin-dependent protein kinase I-gamma antibody
Calcium/calmodulin-dependent protein kinase Ig antibody
Western blot - Anti-CaMKI gamma antibody (ab38132)
Anti-CaMKI gamma antibody (ab38132) at 1 µg/ml + Human recombinant CAMKI gamma kinase at 1 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
79 kDa (
why is the actual band size different from the predicted?
Recombinant CAMKI gamma kinase is a fusion protein with a molecular weight of 79 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-CaMKI gamma antibody (ab38132)
ICC/IF image of ab38132 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab38132, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"