Anti-CaMKI (phospho T177) antibody (ab62215)

Overview

  • Product nameAnti-CaMKI (phospho T177) antibody
    See all CaMKI primary antibodies
  • Description
    Rabbit polyclonal to CaMKI (phospho T177)
  • Specificityab62215 detects CaMKI only when phosphorylated at threonine 177.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human CaMKI around the phosphorylation site of threonine 177 (L-S-TP-A-C).

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • FunctionCalcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
  • Tissue specificityWidely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • DomainThe autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • Post-translational
    modifications
    Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
    Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium / calmodulin dependent protein kinase 1 antibody
    • Calcium / calmodulin dependent protein kinase I antibody
    • Calcium / calmodulin dependent protein kinase type 1 antibody
    • Calcium/calmodulin dependent protein kinase 1 antibody
    • Calcium/calmodulin dependent protein kinase I antibody
    • Calcium/calmodulin dependent protein kinase type 1 antibody
    • Calcium/calmodulin-dependent protein kinase type 1 antibody
    • CaM K1 antibody
    • CaM KI antibody
    • CaM kinase 1 alpha antibody
    • CAM kinase 1 antibody
    • CaM kinase I alpha antibody
    • CaM kinase I antibody
    • CaM-KI antibody
    • CaMK 1 antibody
    • CAMK I antibody
    • CaMK1 alpha antibody
    • CAMK1 antibody
    • CAMK1 PEN antibody
    • CaMKI alpha antibody
    • CaMKI-alpha antibody
    • KCC1A_HUMAN antibody
    • MGC120317 antibody
    • MGC120318 antibody
    see all

Anti-CaMKI (phospho T177) antibody images

  • Immunofluorescence analysis of HeLa cells, using ab62215 (1/500 - 1/1000) staining CaMKI; in the presence and absence of immunizing peptide.
  • Ab62215 staining human normal occipital cortex. Staining is localised to nuclear and cytoplasmic compartments.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-CaMKI (phospho T177) antibody (ab62215)

ab62215 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (failing and nonfailing cardiac tissue, mouse and h)
Loading amount 45 µg
Specification failing and nonfailing cardiac tissue, mouse and h
Gel Running Conditions Non-reduced Denaturing (Biorad Criterion 7.5 and 15% 150 volts)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Dec 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"