Overview

  • Product nameAnti-CaMKII alpha antibody
    See all CaMKII alpha primary antibodies
  • Description
    Rabbit polyclonal to CaMKII alpha
  • Specificityab50202 does not cross-react with g and d isoforms (60 kDa). This antibody does cross-react with CAMKII beta protein
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide:

    KWQIVHFHRSGAPSVLPH

    conjugated to KLH, corresponding to C terminal amino acids 461-478 of Rat CaMKII alpha

  • Positive control
    • Synaptosomal fraction of rat brain

Properties

Applications

Our Abpromise guarantee covers the use of ab50202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 54 kDa. Staining of CaM Kinase IIa in immunoblotting is specifically inhibited with CaM Kinase IIa immunizing peptide.
IP Use at an assay dependent dilution. 5 µg using 10 µg of a synaptosomal fraction of rat brain.

Target

  • FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaMK II alpha subunit antibody
    • CaMK-II subunit alpha antibody
    • Camk2a antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • EC 2.7.11.17 antibody
    • KCC2A_HUMAN antibody
    • KIAA0968 antibody
    • MGC123320 antibody
    • MGC139375 antibody
    • MGC155201 antibody
    • mKIAA0968 antibody
    • PK2CDD antibody
    • PKCCD antibody
    • R74975 antibody
    • zgc:112538 antibody
    • zgc:123320 antibody
    see all

References for Anti-CaMKII alpha antibody (ab50202)

This product has been referenced in:
  • Thein S  et al. CaMKII mediates recruitment and activation of the deubiquitinase CYLD at the postsynaptic density. PLoS One 9:e91312 (2014). WB ; Rat . Read more (PubMed: 24614225) »
  • Dumoulin MC  et al. Extracellular Signal-Regulated Kinase (ERK) Activity During Sleep Consolidates Cortical Plasticity In Vivo. Cereb Cortex N/A:N/A (2013). WB ; Cat . Read more (PubMed: 24047601) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 2 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Rat Tissue lysate - whole (hippocampus,tissue lysate)
Specification hippocampus,tissue lysate
Treatment RIPA
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Jun 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Christiane Rewerts

Verified customer

Submitted Mar 31 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"