Overview

  • Product nameAnti-CaMKII alpha antibody
    See all CaMKII alpha primary antibodies
  • Description
    Goat polyclonal to CaMKII alpha
  • SpecificityExpected to recognize both reported isoforms of CaMKII (NP_057065.2 and NP_741960.1). This antibody does cross-react with CAMKII beta protein
  • Tested applicationsSuitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Peptide with sequence

    C-PRTAQSEETRVWHR

    , corresponding to internal sequence amino acids 445-458 of human CaMKII alpha protein (NP_057065.2; NP_741960.1). (Peptide available as ab126462.)

  • Positive control
    • Human brain (cerebellum) and mouse brain lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55, 65 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human CaMKII alpha peptide (ab126462).
IHC-FoFr Use at an assay dependent concentration. PubMed: 24231734

Target

  • FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaMK II alpha subunit antibody
    • CaMK-II subunit alpha antibody
    • Camk2a antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • EC 2.7.11.17 antibody
    • KCC2A_HUMAN antibody
    • KIAA0968 antibody
    • MGC123320 antibody
    • MGC139375 antibody
    • MGC155201 antibody
    • mKIAA0968 antibody
    • PK2CDD antibody
    • PKCCD antibody
    • R74975 antibody
    • zgc:112538 antibody
    • zgc:123320 antibody
    see all

Anti-CaMKII alpha antibody images

  • Anti-CaMKII alpha antibody (ab87597) at 0.1 µg/ml + mouse brain lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab87597 at 3.8 µg/ml staining CaMKII in Human brain cortex. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

References for Anti-CaMKII alpha antibody (ab87597)

This product has been referenced in:
  • Gan JO  et al. Adolescent social isolation enhances the plasmalemmal density of NMDA NR1 subunits in dendritic spines of principal neurons in the basolateral amygdala of adult mice. Neuroscience 258:174-83 (2014). IHC-FoFr ; Mouse . Read more (PubMed: 24231734) »
  • Zhong W  et al. Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva). PLoS One 9:e104718 (2014). IHC-Fr . Read more (PubMed: 25121483) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 30 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Cat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 13 2012

I am very pleased to hear you would like to accept our offer and test ab87597 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in th...

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Thank you very much for your interest in ab87597.

To our knowledge, ab87597 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab87597 now, test it in IHC-Fr and submit feedback to us in the f...

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Vielen Dank für Ihre Anfrage.

Ich kann empfehlen den ab87597 mit einer Verdünnungreihe, ausgehend von 1/100 )zum Beispiel 1/50, 1/100 und 1/200) auszutested. Auch kann ich empfehlen, die Antigendemaskierung mit einem Citrate Puffer pH 6 unte...

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Thank you for contacting Abcam yesterday.

I have talked to the lab and we will be able to get the blocking peptide for ab87597 added to our catalogue. The product should be added in a day or so, therefore you should be able to purchase it by ...

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