Rat Predicted to work with:
Mouse, Rabbit, Chicken, Cow, Human, Pig, Orangutan
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CaMKII alpha + CaMKII beta (phospho T286) aa 250-350 conjugated to Keyhole Limpet Haemocyanin (KLH). (Peptide available as ab155646, ab115237, ab115237)
This antibody gave a positive signal in rat brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
RelevanceFunction: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Tissue specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
Western blot - Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody (ab91290)
All lanes : Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody (ab91290) at 1/250 dilution
Lane 1 : Brain (Rat) Tissue Lysate Lane 2 : Brain (Rat) Tissue Lysate with CaMKII alpha (phospho T286) peptide (ab115237) at 1 µg/ml Lane 3 : Brain (Rat) Tissue Lysate with Human CaMKII alpha peptide (ab155646) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 54 kDa Observed band size : 54 kDa Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.