Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody (ab91290)

Overview

  • Product nameAnti-CaMKII alpha + CaMKII beta (phospho T286) antibody
    See all CaMKII alpha + CaMKII beta (phospho T286) primary antibodies
  • Description
    Rabbit polyclonal to CaMKII alpha + CaMKII beta (phospho T286)
  • SpecificityDue to the homology between the immunogen peptide and other CAMKII isoforms, ab124880 may cross react with CAMKII delta (KCC2D, 100% homology)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Chicken, Cow, Human, Pig, Orangutan
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CaMKII alpha + CaMKII beta (phospho T286) aa 250-350 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab155646, ab115237, ab115237)

  • Positive control
    • This antibody gave a positive signal in rat brain tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab91290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

Target

  • RelevanceFunction: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Tissue specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
  • Database links
  • Alternative names
    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
    • Calcium/calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II beta antibody
    • Calcium/calmodulin dependent protein kinase IIB antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin dependent protein kinase type II beta chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II antibody
    • calcium/calmodulin-dependent protein kinase II beta antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CAM 2 antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II antibody
    • CaM kinase II beta chain antibody
    • CaM kinase II beta subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaM kinase II subunit beta antibody
    • CaM-kinase II beta chain antibody
    • CAM2 antibody
    • CAMK 2 antibody
    • CaMK II alpha subunit antibody
    • CaMK II beta subunit antibody
    • CaMK-II antibody
    • CaMK-II subunit alpha antibody
    • CaMK-II subunit beta antibody
    • Camk2 antibody
    • Camk2a antibody
    • Camk2b antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • KCC2_HUMAN antibody
    • KCC2A_HUMAN antibody
    • KCC2B_HUMAN antibody
    see all

Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody images

  • All lanes : Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody (ab91290) at 1/250 dilution

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate with CaMKII alpha (phospho T286) peptide (ab115237) at 1 µg/ml
    Lane 3 : Brain (Rat) Tissue Lysate with Human CaMKII alpha peptide (ab155646) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-CaMKII alpha + CaMKII beta (phospho T286) antibody (ab91290)

ab91290 has not yet been referenced specifically in any publications.

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