• Product nameAnti-CaMKII antibody [6G9]
    See all CaMKII primary antibodies
  • Description
    Mouse monoclonal [6G9] to CaMKII
  • SpecificityThis antibody detects the CaMKII alpha subunit in both phosphorylated and non phosphorylated forms. Expected reactivity also includes the delta and gamma isoforms but not the beta isoform.
  • Tested applicationsSuitable for: IHC-Fr, IP, IHC-P, ICC/IF, WB, RIA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Partially purified full length native rat protein.

  • Positive control
    • WB: Human, rat and mouse whole brain tissue lysates. Flow Cyt: PC12 cells. IHC - P: Rat normal brain FFPE tissue sections.
  • General notes

    Alternative versions available:

    Anti-CaMKII antibody (Alexa Fluor® 647) [6G9] (ab197499)

    Anti-CaMKII antibody (HRP) [6G9] (ab197500)



Our Abpromise guarantee covers the use of ab22609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22201125
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
RIA Use a concentration of 3 - 5 µg/ml.
ELISA Use at an assay dependent concentration.


Anti-CaMKII antibody [6G9] images

  • IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 20 µg per lane.

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

References for Anti-CaMKII antibody [6G9] (ab22609)

This product has been referenced in:
  • Geng KW  et al. Ethanol Increases Mechanical Pain Sensitivity in Rats via Activation of GABAA Receptors in Medial Prefrontal Cortex. Neurosci Bull N/A:N/A (2016). IF . Read more (PubMed: 27628528) »
  • Iwabuchi M  et al. Characterization of the ubiquitin-modified proteome regulated by transient forebrain ischemia. J Cereb Blood Flow Metab 34:425-32 (2014). Read more (PubMed: 24301296) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Dorsal Raphe Nucleus)
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton X-100
Specification Dorsal Raphe Nucleus
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Formalin

Abcam user community

Verified customer

Submitted Oct 26 2015

Thank you for contacting us.

The Bovine species for all of these antibodies is predicted. I am sorry we do not hold any bovine specific data.

The immunogen of these antibodies are as follows;

- ab22609; Partially purified ful...

Read More

Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

Read More