Recombinant Anti-CaMKII antibody [EP1829Y] (ab52476)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1829Y] to CaMKII
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-CaMKII antibody [EP1829Y]
See all CaMKII primary antibodies -
Description
Rabbit monoclonal [EP1829Y] to CaMKII -
Host species
Rabbit -
Specificity
The peptide immunogen is highly conserved between CaMKII alpha, beta, gamma, and delta. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CaMKII aa 200-300. The exact sequence is proprietary.
(Peptide available asab199551) -
Positive control
- WB: Mouse brain, rat brain and human fetal brain tisse lysates. IHC-P: Human hepatocellulas carcinoma tissue. ICC/IF: U87-MG and PC12 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1829Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- HRP Anti-CaMKII antibody [EP1829Y] (ab195518)
- Alexa Fluor® 647 Anti-CaMKII antibody [EP1829Y] (ab196165)
- Alexa Fluor® 488 Anti-CaMKII antibody [EP1829Y] (ab196378)
- Alexa Fluor® 555 Anti-CaMKII antibody [EP1829Y] (ab206883)
- Anti-CaMKII antibody [EP1829Y] - Low endotoxin, Azide free (ab219365)
- Anti-CaMKII antibody [EP1829Y] - BSA and Azide free (ab271841)
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/250.
|
|
WB | (7) |
1/1000 - 1/5000. Detects a band of approximately 45, 70 kDa (predicted molecular weight: 54 kDa).
For unpurified use at 1/20000. |
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
1/250. |
WB
1/1000 - 1/5000. Detects a band of approximately 45, 70 kDa (predicted molecular weight: 54 kDa). For unpurified use at 1/20000. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
-
Database links
- Entrez Gene: 815 Human
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12322 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
see all -
Alternative names
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
see all
Images
-
Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 45,70 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
All lanes : Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 45,70 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/20000 dilution (unpurified) + Mouse brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 45,70 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CaMKII with purified ab52476 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
-
Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling CaMKII with purified ab52476 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% aTriton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
-
ICC/IF image of unpurified ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52476, 1µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (99)
ab52476 has been referenced in 99 publications.
- Zhang XZ et al. Insulin-like growth factor-1 inhibits apoptosis of rat gastric smooth muscle cells under high glucose condition via adenosine monophosphate-activated protein kinase (AMPK) pathway. Folia Histochem Cytobiol 60:74-88 (2022). PubMed: 35156189
- Wei B et al. T1AM Attenuates the Hypoxia/Reoxygenation-Induced Necroptosis of H9C2 Cardiomyocytes via RIPK1/RIPK3 Pathway. Biomed Res Int 2022:4833791 (2022). PubMed: 35265713
- Zhang Y et al. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/β-catenin signaling in obstructed kidneys in vivo. J Biol Chem 298:102010 (2022). PubMed: 35525270
- Yu J et al. Effects and potential mechanism of Ca2+/calmodulin‑dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle. Int J Mol Med 50:N/A (2022). PubMed: 35929517
- Mi S et al. CaMKII is a modulator in neurodegenerative diseases and mediates the effect of androgen on synaptic protein PSD95. Front Genet 13:959360 (2022). PubMed: 35991539