This antibody gave a positive signal in PC12 whole cell lysate as well as the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Forebrain; Rat Cortex; Human Skeletal muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 72 kDa).
FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Tissue specificityWidely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
FormThe multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
Calcium/calmodulin dependent protein kinase II beta antibody
Calcium/calmodulin dependent protein kinase IIB antibody
Calcium/calmodulin dependent protein kinase type II beta chain antibody
calcium/calmodulin-dependent protein kinase II beta antibody
Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
CAM 2 antibody
CaM kinase II beta chain antibody
CaM kinase II beta subunit antibody
CaM kinase II subunit beta antibody
CaM-kinase II beta chain antibody
CAMK 2 antibody
CAMK 2B antibody
CaMK II beta subunit antibody
CaMK II subunit beta antibody
CaMK-II subunit beta antibody
CaMK2 beta antibody
CaMKII beta subunit antibody
Proline rich calmodulin dependent protein kinase antibody
proline rich calmodulin-dependent protein kinase antibody
Anti-CaMKII beta antibody images
Western blot - Anti-CaMKII antibody (ab107163)
All lanes : Anti-CaMKII beta antibody (ab107163) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lane 4 : Forebrain (Mouse) Tissue Lysate Lane 5 : Rat Cortex Tissue Lysate Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique