- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CaMKII beta antibody
See all CaMKII beta primary antibodies -
DescriptionRabbit polyclonal to CaMKII beta
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Host speciesRabbit
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SpecificityThis antibody does cross-react with CAMKII alpha protein
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Macaque monkey, Gorilla
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CaMKII.
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Positive control
- This antibody gave a positive signal in PC12 whole cell lysate as well as the following tissue lysates: Human Brain; Mouse Brain; Rat Brain; Mouse Forebrain; Rat Cortex; Human Skeletal muscle.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab107163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 72 kDa). |
Target
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FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
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Tissue specificityWidely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
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Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 816 Human
- Entrez Gene: 12323 Mouse
- Entrez Gene: 24245 Rat
- Omim: 607707 Human
- SwissProt: Q13554 Human
- SwissProt: P28652 Mouse
- SwissProt: P08413 Rat
- Unigene: 351887 Human
see all -
FormThe multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
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Alternative names
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase IIB antibody
see all
Images
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Western blot - Anti-CaMKII antibody (ab107163)All lanes : Anti-CaMKII beta antibody (ab107163) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Forebrain (Mouse) Tissue Lysate
Lane 5 : Rat Cortex Tissue Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 65 kDa (why is the actual band size different from the predicted?)
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Protocols
References
ab107163 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"