Overview

  • Product nameAnti-CaMKII beta antibody
    See all CaMKII beta primary antibodies
  • Description
    Rabbit polyclonal to CaMKII beta
  • SpecificityThis antibody does cross-react with CAMKII alpha protein
  • Tested applicationsSuitable for: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse CaMKII beta.

    (Peptide available as ab27843.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue Brain (Rat) Tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.

Properties

Applications

Our Abpromise guarantee covers the use of ab34703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Mouse CaMKII beta peptide (ab27843).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • FunctionCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • Tissue specificityWidely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • FormThe multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
  • Alternative names
    • Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
    • Calcium/calmodulin dependent protein kinase II beta antibody
    • Calcium/calmodulin dependent protein kinase IIB antibody
    • Calcium/calmodulin dependent protein kinase type II beta chain antibody
    • calcium/calmodulin-dependent protein kinase II beta antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
    • CAM 2 antibody
    • CaM kinase II beta chain antibody
    • CaM kinase II beta subunit antibody
    • CaM kinase II subunit beta antibody
    • CaM-kinase II beta chain antibody
    • CAM2 antibody
    • CAMK 2 antibody
    • CAMK 2B antibody
    • CaMK II beta subunit antibody
    • CaMK II subunit beta antibody
    • CaMK-II subunit beta antibody
    • CAMK2 antibody
    • CaMK2 beta antibody
    • Camk2b antibody
    • CAMKB antibody
    • CaMKII beta subunit antibody
    • CaMKIIB antibody
    • KCC2B_HUMAN antibody
    • MGC29528 antibody
    • Proline rich calmodulin dependent protein kinase antibody
    • proline rich calmodulin-dependent protein kinase antibody
    see all

Anti-CaMKII beta antibody images

  • All lanes : Anti-CaMKII beta antibody (ab34703) at 1 µg/ml

    Lane 1 : Brain Mouse Tissue Lysate
    Lane 2 : Brain Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab34703 staining CaMKII in rat brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgGwas used as secondary at 1/1000 dilution.

    See Abreview

  • ab34703 staining CaMKII beta in adult mouse cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 0.01M Tris HCL, pH 10. Samples were then permeabilized using 0.3% Triton X-100, blocked with 5% BSA for 45 minutes at 22°C and then incubated with ab34703 at a 1/3000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/2000 dilution.

    Strong siganls were detected in molecular layers (M), and Purkinje cells (P) were positively stained as well. In granule layers (G), some neurons were also strongly stained.

    See Abreview

  • ICC/IF image of ab34703 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34703 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CaMKII beta antibody (ab34703)

This product has been referenced in:
  • Li Q  et al. The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation. Elife 3:e01201 (2014). WB ; Mouse . Read more (PubMed: 24448406) »
  • Ferhatovic L  et al. Expression of calcium/calmodulin-dependent protein kinase II and pain-related behavior in rat models of type 1 and type 2 diabetes. Anesth Analg 116:712-21 (2013). IHC-FoFr ; Rat . Read more (PubMed: 23400989) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Non-reduced Denaturing
Sample Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification hippocampus tissue lysate
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Jun 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse whole brain)
Loading amount 10 µg
Specification mouse whole brain
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse cerebellum)
Specification mouse cerebellum
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M Tris HCL, pH 10
Permeabilization Yes - 0.3% Triton X-100
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Mar 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Mar 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"