This antibody gave a positive signal in the following lysates:
Brain (Mouse) Tissue
Brain (Rat) Tissue
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
Calcium/calmodulin dependent protein kinase II beta antibody
Calcium/calmodulin dependent protein kinase IIB antibody
Calcium/calmodulin dependent protein kinase type II beta chain antibody
calcium/calmodulin-dependent protein kinase II beta antibody
Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
CAM 2 antibody
CaM kinase II beta chain antibody
CaM kinase II beta subunit antibody
CaM kinase II subunit beta antibody
CaM-kinase II beta chain antibody
CAMK 2 antibody
CAMK 2B antibody
CaMK II beta subunit antibody
CaMK II subunit beta antibody
CaMK-II subunit beta antibody
CaMK2 beta antibody
CaMKII beta subunit antibody
Proline rich calmodulin dependent protein kinase antibody
proline rich calmodulin-dependent protein kinase antibody
Western blot - CaMKII beta antibody (ab34703)
All lanes : Anti-CaMKII beta antibody (ab34703) at 1 µg/ml
Lane 1 : Brain Mouse Tissue Lysate Lane 2 : Brain Rat Tissue Lysate
Predicted band size : 60 kDa Observed band size : 60 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - CaMKII beta antibody (ab34703)This image is courtesy of an Abreview submitted by Dr Sophie Pezet
ab34703 staining CaMKII in rat brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgGwas used as secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII beta antibody (ab34703)Image courtesy of an anonymous Abreview.
ab34703 staining CaMKII beta in adult mouse cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 0.01M Tris HCL, pH 10. Samples were then permeabilized using 0.3% Triton X-100, blocked with 5% BSA for 45 minutes at 22°C and then incubated with ab34703 at a 1/3000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/2000 dilution. Strong siganls were detected in molecular layers (M), and Purkinje cells (P) were positively stained as well. In granule layers (G), some neurons were also strongly stained.
ICC/IF image of ab34703 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34703 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Kool MJ et al. The molecular, temporal and region-specific requirements of the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in mouse locomotion. Sci Rep6:26989 (2016).
Read more (PubMed: 27244486) »