Validated using a knockout cell line
Recombinant
RabMAb

Anti-CaMKII delta antibody [EPR13095] (ab181052)

Overview

  • Product name
    Anti-CaMKII delta antibody [EPR13095]
    See all CaMKII delta primary antibodies
  • Description
    Rabbit monoclonal [EPR13095] to CaMKII delta
  • Host species
    Rabbit
  • Specificity
    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CaMKII delta aa 1-100. The exact sequence is proprietary.
    Database link: Q13557
    (Peptide available as ab188479)

  • Positive control
    • SW480, A431 and HeLa whole cell lysate; Human skeletal muscle tissue; SH-SY5Y; Mouse heart; Rat spleen; Human cardiac; Human thyroid .
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).Can be blocked with CaMKII delta peptide (ab188479).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
    • Tissue specificity
      Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Calcium / calmodulin dependent protein kinase 2 delta antibody
      • Calcium / calmodulin dependent protein kinase II delta antibody
      • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
      • calcium/calmodulin-dependent protein kinase type II delta chain antibody
      • Calcium/calmodulin-dependent protein kinase type II subunit delta antibody
      • CAM kinase 2 delta antibody
      • CAM kinase II delta antibody
      • CaM kinase II delta subunit antibody
      • CaM kinase II subunit delta antibody
      • CaM-kinase II delta chain antibody
      • CAMK 2d antibody
      • CaMK-II delta subunit antibody
      • CaMK-II subunit delta antibody
      • CAMK2D antibody
      • CAMKD antibody
      • CAMKI antibody
      • KCC2D_HUMAN antibody
      • RATCAMKI antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
    • Lane 1 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution
      Lanes 2-4 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution (purified)

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg
      Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
      Lane 3 : Mouse heart lysates at 20 µg
      Lane 4 : Rat spleen lysates at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 56 kDa



      Blocking and diluting buffer : 5% NFDM/TBST

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CaMKII delta knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Unpurified ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/2000 dilution (unpurified)

      Lane 1 : SW480 whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 56 kDa



      Blocking/ Dilution buffer: 5% NFDM /TBST.

    • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using unpurified ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    References

    This product has been referenced in:
    • Wu Y  et al. Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation. Biomed Res Int 2017:4150158 (2017). Read more (PubMed: 28536695) »
    • Liu L  et al. The H19 long noncoding RNA is a novel negative regulator of cardiomyocyte hypertrophy. Cardiovasc Res 111:56-65 (2016). Read more (PubMed: 27084844) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse Renal - Including KO)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Mouse Renal - Including KO
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mr. Edward Humphries

    Verified customer

    Submitted May 24 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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