Validated using a knockout cell line
Recombinant
RabMAb

Anti-CaMKII delta antibody [EPR13095] (ab181052)

Overview

  • Product name
    Anti-CaMKII delta antibody [EPR13095]
    See all CaMKII delta primary antibodies
  • Description
    Rabbit monoclonal [EPR13095] to CaMKII delta
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CaMKII delta aa 1-100. The exact sequence is proprietary.
    Database link: Q13557

  • Positive control
    • SW480, A431 and HeLa whole cell lysate; Human skeletal muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).Can be blocked with CaMKII delta peptide (ab188479).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.
  • Tissue specificity
    Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium / calmodulin dependent protein kinase 2 delta antibody
    • Calcium / calmodulin dependent protein kinase II delta antibody
    • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
    • calcium/calmodulin-dependent protein kinase type II delta chain antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit delta antibody
    • CAM kinase 2 delta antibody
    • CAM kinase II delta antibody
    • CaM kinase II delta subunit antibody
    • CaM kinase II subunit delta antibody
    • CaM-kinase II delta chain antibody
    • CAMK 2d antibody
    • CaMK-II delta subunit antibody
    • CaMK-II subunit delta antibody
    • CAMK2D antibody
    • CAMKD antibody
    • CAMKI antibody
    • KCC2D_HUMAN antibody
    • RATCAMKI antibody
    see all

Images



  • Predicted band size : 56 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CaMKII delta knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/2000 dilution

    Lane 1 : SW480 whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 56 kDa

    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

References

This product has been referenced in:
  • Wu Y  et al. Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation. Biomed Res Int 2017:4150158 (2017). Read more (PubMed: 28536695) »
  • Liu L  et al. The H19 long noncoding RNA is a novel negative regulator of cardiomyocyte hypertrophy. Cardiovasc Res 111:56-65 (2016). Read more (PubMed: 27084844) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Renal - Including KO)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Mouse Renal - Including KO
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Edward Humphries

Verified customer

Submitted May 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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