Overview

  • Product name
    Anti-CamKII gamma antibody
    See all CamKII gamma primary antibodies
  • Description
    Rabbit polyclonal to CamKII gamma
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human CamKII gamma aa 309-338 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • SKBR3 cell lysate, mouse heart tissue lysate, human hepatocarcinoma This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH

Properties

Applications

Our Abpromise guarantee covers the use of ab37999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/50 - 1/100.

Target

Images

  • ICC/IF image of ab37999 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37999 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CamKII gamma antibody (ab37999) at 1/1000 dilution + mouse heart tissue lysate at 35 µg/ml

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 88 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab37999 at 1/100 dilution staining CamKII in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by AEC staining.

References

This product has been referenced in:
  • Chakraborty A  et al. Inhibition of CaMKII does not attenuate cardiac hypertrophy in mice with dysfunctional ryanodine receptor. PLoS One 9:e104338 (2014). WB ; Mouse . Read more (PubMed: 25093823) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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