CAMKIV 293T Transfected Lysate - (positive control) (ab94283)

Overview

  • Product nameCAMKIV 293T Transfected Lysate - (positive control)
  • General notesab94283 is a 293T cell transfected lysate in which Human CAMKIV has been transiently over-expressed using a pCMV-CAMKIV plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory. Tissue specificity: Expressed in epithelial ovarian cancer tissue. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. PTM: Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.

Applications

Our Abpromise guarantee covers the use of ab94283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • CAMKIV 293T Transfected Lysate - (positive control) images

    • ab94283 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-CAMKIV antibody (ab88544) at 1/500 dilution

      Lane 1 : CAMKIV 293T Transfected Lysate - (positive control) (ab94283)
      Lane 2 : 293T Non Transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for CAMKIV 293T Transfected Lysate - (positive control) (ab94283)

    ab94283 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"