Overview

  • Product name
  • Description
    Rabbit polyclonal to CAMKIV
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide:

    ANFVHMDTAQKKLQEFNAR

    , corresponding to amino acids 305-323 of Human CAMKIV

  • Positive control
    • Rat brain or Jurkat cells lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab34868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA 1/5000 - 1/25000.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
  • Tissue specificity
    Expressed in epithelial ovarian cancer tissue.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
  • Cellular localization
    Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
    • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
    • Brain Ca++-calmodulin dependent protein kinase type IV antibody
    • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
    • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
    • Calcium/calmodulin dependent protein kinase IV antibody
    • Calcium/calmodulin dependent protein kinase type IV antibody
    • Calcium/calmodulin-dependent protein kinase type IV antibody
    • CAM kinase 4 antibody
    • CAM kinase GR antibody
    • CAM kinase IV antibody
    • CAM kinase-GR antibody
    • CaMK 4 antibody
    • CAMK GR antibody
    • CaMK IV antibody
    • Camk4 antibody
    • CaMKGR antibody
    • IV antibody
    • KCC4_HUMAN antibody
    • MGC36771 antibody
    see all

Images

  • All lanes : Anti-CAMKIV antibody (ab34868) at 1/1000 dilution (for 2 hours at room temperature)

    Lane 1 : Rat brain lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Rat brain lysate with Immunizing peptide
    Lane 4 : Jurkat cells lysate with Immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Secondary
    IRDye TM800 conjugated Gt-a-Rabbit IgG for 45 min at room temperature at 1/10000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References

ab34868 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab34868.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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