Overview

  • Product nameAnti-CAMKIV antibody
    See all CAMKIV primary antibodies
  • Description
    Rabbit polyclonal to CAMKIV
  • Tested applicationsSuitable for: IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Xenopus laevis, Non Human Primates, Plants, Turtle
  • Immunogen

    Synthetic peptide corresponding to Mouse CAMKIV aa 127-143.
    Sequence:

    VEKGYYSERDAADAVKQ


    (Peptide available as ab5859)

  • Positive control
    • WB: HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).Can be blocked with CAMKIV peptide (ab5859).

This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate.

ICC/IF Use at an assay dependent concentration. PubMed: 18802105

Target

  • FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
  • Tissue specificityExpressed in epithelial ovarian cancer tissue.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
  • Cellular localizationCytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
    • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
    • Brain Ca++-calmodulin dependent protein kinase type IV antibody
    • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
    • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
    • Calcium/calmodulin dependent protein kinase IV antibody
    • Calcium/calmodulin dependent protein kinase type IV antibody
    • Calcium/calmodulin-dependent protein kinase type IV antibody
    • CAM kinase 4 antibody
    • CAM kinase GR antibody
    • CAM kinase IV antibody
    • CAM kinase-GR antibody
    • CaMK 4 antibody
    • CAMK GR antibody
    • CaMK IV antibody
    • Camk4 antibody
    • CaMKGR antibody
    • IV antibody
    • KCC4_HUMAN antibody
    • MGC36771 antibody
    see all

Anti-CAMKIV antibody images



  • Predicted band size : 52 kDa

    ab3557 at a 0.5µg/ml concentration staining 55 kDa CaMKIV in rat brain extract by Western blot.

References for Anti-CAMKIV antibody (ab3557)

This product has been referenced in:
  • Zheng Z  et al. Oligomeric amyloid-{beta} inhibits the proteolytic conversion of brain-derived neurotrophic factor (BDNF), AMPA receptor trafficking, and classical conditioning. J Biol Chem 285:34708-17 (2010). WB ; Turtle . Read more (PubMed: 20807770) »
  • Zheng Z & Keifer J PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning. J Neurophysiol 101:2539-49 (2009). WB ; Turtle . Read more (PubMed: 19261706) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (EL4)
Loading amount 10000 µg
Specification EL4
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2012

Application Western blot
Sample Zebrafish Tissue lysate - whole (ZF embryo)
Loading amount 30 µg
Specification ZF embryo
Treatment de-yolked
Gel Running Conditions Non-reduced Denaturing (10% SDS)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Ms. Gemma Lace

Verified customer

Submitted Jan 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (huh-7 cells)
Loading amount 10000 cells
Specification huh-7 cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"