Overview

  • Product nameAnti-CAMKIV antibody
    See all CAMKIV primary antibodies
  • Description
    Rabbit polyclonal to CAMKIV
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 425 and the C-terminus (residue 473) of human CAMKIV (Swiss-Prot Q16566).

  • Positive control
    • Whole cell lysate from Jurkat and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/15000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP Use at 3-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000.

Target

  • FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
  • Tissue specificityExpressed in epithelial ovarian cancer tissue.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
  • Cellular localizationCytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
    • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
    • Brain Ca++-calmodulin dependent protein kinase type IV antibody
    • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
    • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
    • Calcium/calmodulin dependent protein kinase IV antibody
    • Calcium/calmodulin dependent protein kinase type IV antibody
    • Calcium/calmodulin-dependent protein kinase type IV antibody
    • CAM kinase 4 antibody
    • CAM kinase GR antibody
    • CAM kinase IV antibody
    • CAM kinase-GR antibody
    • CaMK 4 antibody
    • CAMK GR antibody
    • CaMK IV antibody
    • Camk4 antibody
    • CaMKGR antibody
    • IV antibody
    • KCC4_HUMAN antibody
    • MGC36771 antibody
    see all

Anti-CAMKIV antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CAMKIV with ab70229 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-CAMKIV antibody (ab70229) at 0.04 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg


    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Lane3: 250µg Jurkat whole cell lysate immunoprecipitated with ab70229 at 3 µg/mg lysate Lane4: 250µg Jurkat whole cell lysate with IgG control. All lanes blotted with ab70229 at 0.04 µg/ml. Lane 1 and Lane 2: samples immunoprecipitated with irrelevant antibodies.

References for Anti-CAMKIV antibody (ab70229)

ab70229 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70229.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"