Overview

  • Product name
    Anti-CAMKIV antibody [EP2564Y]
    See all CAMKIV primary antibodies
  • Description
    Rabbit monoclonal [EP2564Y] to CAMKIV
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CAMKIV aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • Jurkat cell lysate and human brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab75874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
    • Tissue specificity
      Expressed in epithelial ovarian cancer tissue.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
    • Cellular localization
      Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
      • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
      • Brain Ca++-calmodulin dependent protein kinase type IV antibody
      • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
      • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
      • Calcium/calmodulin dependent protein kinase IV antibody
      • Calcium/calmodulin dependent protein kinase type IV antibody
      • Calcium/calmodulin-dependent protein kinase type IV antibody
      • CAM kinase 4 antibody
      • CAM kinase GR antibody
      • CAM kinase IV antibody
      • CAM kinase-GR antibody
      • CaMK 4 antibody
      • CAMK GR antibody
      • CaMK IV antibody
      • Camk4 antibody
      • CaMKGR antibody
      • IV antibody
      • KCC4_HUMAN antibody
      • MGC36771 antibody
      see all

    Images

    • All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 60 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27611779) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your reply.
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