Overview

  • Product nameAnti-CAMKIV antibody [EP2564Y]
    See all CAMKIV primary antibodies
  • Description
    Rabbit monoclonal [EP2564Y] to CAMKIV
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the N terminal region of human CaMKIV.

  • Positive control
    • Jurkat cell lysate and human brain lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab75874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
    • Tissue specificityExpressed in epithelial ovarian cancer tissue.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
    • Cellular localizationCytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
      • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
      • Brain Ca++-calmodulin dependent protein kinase type IV antibody
      • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
      • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
      • Calcium/calmodulin dependent protein kinase IV antibody
      • Calcium/calmodulin dependent protein kinase type IV antibody
      • Calcium/calmodulin-dependent protein kinase type IV antibody
      • CAM kinase 4 antibody
      • CAM kinase GR antibody
      • CAM kinase IV antibody
      • CAM kinase-GR antibody
      • CaMK 4 antibody
      • CAMK GR antibody
      • CaMK IV antibody
      • Camk4 antibody
      • CaMKGR antibody
      • IV antibody
      • KCC4_HUMAN antibody
      • MGC36771 antibody
      see all

    Anti-CAMKIV antibody [EP2564Y] images

    • All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 52 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    References for Anti-CAMKIV antibody [EP2564Y] (ab75874)

    This product has been referenced in:
    • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27611779) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"