The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. PubMed: 23593332
1/50 - 1/100.
FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
Tissue specificityExpressed in epithelial ovarian cancer tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
Post-translational modificationsAutophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
Cellular localizationCytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
ab59424, at a 1/50 dilution, staining paraffin embedded human brain tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.
References for Anti-CAMKIV (phospho T196 + T200) antibody (ab59424)
This product has been referenced in:
Moriguchi S et al. Stimulation of the sigma-1 receptor by DHEA enhances synaptic efficacy and neurogenesis in the hippocampal dentate gyrus of olfactory bulbectomized mice. PLoS One8:e60863 (2013).
Read more (PubMed: 23593332) »
Oury F et al. CREB mediates brain serotonin regulation of bone mass through its expression in ventromedial hypothalamic neurons. Genes Dev24:2330-42 (2010).
Read more (PubMed: 20952540) »