Overview

  • Product name
  • Description
    Rabbit polyclonal to CaMKK
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of Human CaMKK.

  • Positive control
    • Mouse thymus tissue lysate. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab80066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Domain
    The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
    The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
  • Post-translational
    modifications
    Appears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ca2+/calmodulin-dependent protein kinase kinase alpha antibody
    • Calcium/calmodulin dependent protein kinase kinase antibody
    • Calcium/calmodulin dependent protein kinase kinase 1 alpha antibody
    • Calcium/calmodulin dependent protein kinase kinase 1 antibody
    • Calcium/calmodulin dependent protein kinase kinase alpha antibody
    • Calcium/calmodulin-dependent protein kinase kinase 1 antibody
    • Calcium/calmodulin-dependent protein kinase kinase alpha antibody
    • CaM kinase kinase alpha antibody
    • CaM-kinase IV kinase antibody
    • CaM-kinase kinase 1 antibody
    • CaM-kinase kinase alpha antibody
    • CaM-KK 1 antibody
    • CaM-KK alpha antibody
    • CaMKK 1 antibody
    • CaMKK alpha antibody
    • CAMKK alpha protein antibody
    • CaMKK antibody
    • Camkk1 antibody
    • CAMKKA antibody
    • DKFZp761M0423 antibody
    • KKCC1_HUMAN antibody
    • MGC34095 antibody
    • OTTMUSP00000006419 antibody
    see all

Images

  • ICC/IF image of ab80066 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80066, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CaMKK antibody (ab80066) at 1/50 dilution + mouse thymus tissue lysate at 35 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

References

ab80066 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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