Anti-CAMKK2 antibody (ab96531)


  • Product nameAnti-CAMKK2 antibody
    See all CAMKK2 primary antibodies
  • Description
    Rabbit polyclonal to CAMKK2
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-15 of CAMKK2 isoform 5 (NP_705720)

  • Positive control
    • H1299 Whole cell lysate or HeLaS3 cell lysates. HeLa cells (IF)



Our Abpromise guarantee covers the use of ab96531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
ICC/IF 1/100 - 1/200.
IHC-FoFr 1/1000.


  • FunctionCalcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.
  • Tissue specificityUbiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • DomainThe autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
    The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
  • Post-translational
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium/calmodulin dependent protein kinase beta antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 beta antibody
    • Calcium/calmodulin dependent protein kinase kinase beta antibody
    • Calcium/calmodulin-dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin-dependent protein kinase kinase beta antibody
    • CaM kinase kinase beta antibody
    • CaM KK beta antibody
    • CaM-kinase kinase 2 antibody
    • CaM-kinase kinase beta antibody
    • CaM-KK 2 antibody
    • CaM-KK beta antibody
    • CaMKK 2 antibody
    • CAMKK antibody
    • CaMKK beta antibody
    • CAMKK beta protein antibody
    • Camkk2 antibody
    • CAMKKB antibody
    • KKCC2_HUMAN antibody
    see all

Anti-CAMKK2 antibody images

  • Immunofluorescence analysis of paraformaldehyde fixed HeLa using ab96531 at 1/200 dilution: (1) without DNA probe; (2) merged with DNA probe.
  • Anti-CAMKK2 antibody (ab96531) at 1/500 dilution (10% SDS PAGE) + H1299 whole cell lysate at 30 µg

    Predicted band size : 55 kDa
  • IHC-FoFr image of CAMKK2 using ab96531 staining rat spinal cord.The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

References for Anti-CAMKK2 antibody (ab96531)

ab96531 has not yet been referenced specifically in any publications.

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I have performed a sequence alignment for LKB1 for Dictyostelium and for human (which is used to raise most of our antibodies.) Unfortunately there is only a total of 33% homology between the two proteins. I have...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization No

Dr. Karine Thibault

Verified customer

Submitted Dec 13 2010