Anti-cAMP antibody (ab832)
Key features and details
- Sheep polyclonal to cAMP
- Suitable for: ELISA, RIA
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-cAMP antibody
See all cAMP primary antibodies -
Description
Sheep polyclonal to cAMP -
Host species
Sheep -
Specificity
This antibody reacts with cyclic AMP and to lesser degrees with other 5’ nucleotides. -
Tested applications
Suitable for: ELISA, RIAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to cAMP conjugated to bovine serum albumin. Succinylated cAMP
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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AP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
This antibody may be used in radioimmunoassays to detect and quantitate cAMP; 50% binding is achieved with a 1:1,000 dilution.
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Notes |
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AP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. This antibody may be used in radioimmunoassays to detect and quantitate cAMP; 50% binding is achieved with a 1:1,000 dilution.
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Target
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Relevance
Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery. -
Cellular localization
Secreted -
Alternative names
- 3' 5' cyclic adenosine monophosphate antibody
- Cyclic adenosine monophosphate antibody
- Cyclic AMP antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab832 has been referenced in 3 publications.
- Wang L et al. Anti-CCL22 increases regulatory T cells in CD4+ T cells of rheumatoid arthritis patients via STAT5 pathway. Exp Ther Med 19:2127-2132 (2020). PubMed: 32104275
- Sweeny L et al. Evaluation of tyrosine receptor kinases in the interactions of head and neck squamous cell carcinoma cells and fibroblasts. Oral Oncol 48:1242-9 (2012). PubMed: 22795534
- Parro V et al. A microbial oasis in the hypersaline Atacama subsurface discovered by a life detector chip: implications for the search for life on Mars. Astrobiology 11:969-96 (2011). PubMed: 22149750