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cAMP Assay Kit (ab65355) provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 µl (or ~ 0.02-2 µM) cAMP samples.
Visit our FAQs page for tips and troubleshooting.
Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of GPCRs.
If you are using fluorometric detection, we recommend cAMP Direct Immunoassay Detection Kit (Fluorometric) (ab138880).
|10X cAMP Assay Buffer||WM||1 x 25ml|
|Acetylating Reagent A||Violet||1 x 750µl|
|Acetylating Reagent B||Black||1 x 1.2ml|
|HRP Developer||Amber||1 x 10ml|
|Neutralizing Buffer||NM||1 x 7.5ml|
|Protein G Coated Plate||1 unit|
|Rabbit Anti-cAMP polyclonal Antibody||Red||1 vial|
|Standard cAMP (10 nmol)||Yellow||1 x 10µl|
Our Abpromise guarantee covers the use of ab65355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Measurement of cAMP in Jurkat and HepG2 cells with ab65355. Assay performed using the kit protocol.
Standard curve (colorimetric): mean of duplicates (+/-SD)