Anti-cAMP Protein Kinase Catalytic subunit antibody [4B12] (HRP) (ab10049)

Overview

  • Product nameAnti-cAMP Protein Kinase Catalytic subunit antibody [4B12] (HRP)
    See all cAMP Protein Kinase Catalytic subunit primary antibodies
  • Description
    Mouse monoclonal [4B12] to cAMP Protein Kinase Catalytic subunit (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with cAMP-dependent protein kinase purified from bovine cardiac muscle.

  • General notesConcentration varies from lot to lot and can be provided on request.

Applications

Our Abpromise guarantee covers the use of ab10049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • FunctionPhosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
  • Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
    Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity.
    Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficienncy.
  • Cellular localizationCytoplasm. Cell membrane. Nucleus. Mitochondrion. Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes and Cell projection, cilium, flagellum. Expressed in the midpiece region of the sperm flagellum.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP dependent protein kinase alpha catalytic subunit antibody
    • cAMP dependent protein kinase beta catalytic subunit antibody
    • cAMP dependent protein kinase catalytic beta subunit isoform 4ab antibody
    • cAMP dependent protein kinase catalytic subunit alpha antibody
    • cAMP dependent protein kinase catalytic subunit alpha, isoform 1 antibody
    • cAMP dependent protein kinase catalytic subunit beta antibody
    • cAMP-dependent protein kinase catalytic subunit alpha antibody
    • KAPCA_HUMAN antibody
    • PKA C alpha antibody
    • PKA C beta antibody
    • PKA C-alpha antibody
    • PKACA antibody
    • PKACB antibody
    • PPNAD4 antibody
    • PRKACA antibody
    • PRKACAA antibody
    • PRKACB antibody
    • Protein kinase A catalytic subunit alpha antibody
    • Protein kinase A catalytic subunit antibody
    • Protein kinase A catalytic subunit beta antibody
    • Protein kinase, cAMP dependent, catalytic, alpha antibody
    • Protein kinase, cAMP dependent, catalytic, beta antibody
    see all

References for Anti-cAMP Protein Kinase Catalytic subunit antibody [4B12] (HRP) (ab10049)

ab10049 has not yet been referenced specifically in any publications.

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