Mouse monoclonal [6D2.1] to cAMP Protein Kinase Catalytic subunit
This antibody strongly reacts with human cAMP Protein Kinase A catalytic alpha subunit, and weakly with cAMP Protein Kinase A gamma subunit (both around 40 kDa). The recognized epitope of cAMP Proteine Kinase A catalytic alpha is identical between man, sheep, pig, ox and dog.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 40 kDa.
Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.
Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity. Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficienncy.
Cytoplasm. Cell membrane. Nucleus. Mitochondrion. Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes and Cell projection, cilium, flagellum. Expressed in the midpiece region of the sperm flagellum.
cAMP dependent protein kinase alpha catalytic subunit antibody
cAMP dependent protein kinase beta catalytic subunit antibody
cAMP dependent protein kinase catalytic beta subunit isoform 4ab antibody
cAMP dependent protein kinase catalytic subunit alpha antibody
cAMP dependent protein kinase catalytic subunit alpha, isoform 1 antibody
cAMP dependent protein kinase catalytic subunit beta antibody
cAMP-dependent protein kinase catalytic subunit alpha antibody
PKA C alpha antibody
PKA C beta antibody
PKA C-alpha antibody
Protein kinase A catalytic subunit alpha antibody
Protein kinase A catalytic subunit antibody
Protein kinase A catalytic subunit beta antibody
Protein kinase, cAMP dependent, catalytic, alpha antibody
Protein kinase, cAMP dependent, catalytic, beta antibody
Dot Blot - cAMP Protein Kinase Catalytic subunit antibody [6D2.1] (ab64596)
Dot Blot analysis of GST and GST-fusion proteins using ab64596 and anti-GST (S-tag-05). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
Lane 1: GST-Akt1
Lane 2: GST-Akt2
Lane 3: GST-Akt3
Lane 4: GST-cAMP Protein Kinase Catalytic subunit alpha
Lane 5: GST-cAMP Protein Kinase Catalytic subunit beta
Lane 6: GST-cAMP Protein Kinase Catalytic subunit gamma
Lane 7: GST-MEK 1
Lane 8: GST