Overview

  • Product name
  • Description
    Rabbit polyclonal to CAMSAP1L1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1439 and 1489 of Human CAMSAP1L1 (EAW91320.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 168 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    CAMSAP1L1 (Calmodulin regulated spectrin associated protein 1 like protein 1) is related to CAMSAP1. Both contain an N terminal CH (calponin homology domain) which is an actin binding domain found in cytoskeletal and signal transduction proteins. The CAMSAP1 family of proteins also contains a unique C terminal CKK (DUF1781) domain which has been found to bind microtubules. The function of CAMSAP1L1 has not been characterized.
  • Database links
  • Alternative names
    • Calmodulin regulated spectrin associated protein 1 like 1 antibody
    • Calmodulin regulated spectrin associated protein 2 antibody
    • CAMSAP2 antibody
    • KIAA1078 antibody
    • MGC150680 antibody
    • MGC150681 antibody
    • RP11 93N17.1 antibody
    see all

Images

  • All lanes : Anti-CAMSAP1L1 antibody (ab86683) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 168 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 71 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Western Blot detection of CAMSAP1L1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86683 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab86683 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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