All tags Cancer Bcl-2: the apoptosis checkpoint family

Bcl-2: the apoptosis checkpoint family

Choose from our selection of top quality products for this family of proteins.

For over 30 years B-cell lymphoma-2 (Bcl-2) family have been investigated in cancer research.  A subset of proteins in this family (Bcl-2, Bcl -XL, Mcl-1) prevent apoptosis, while others (Bax. Bak, Bad, Bim) trigger the cascades leading to programmed cell death (Figure 1). Thus the activity of the Bcl-2 family members can have huge effects on cell survival, making them desirable therapeutic targets.

See below for a breakdown of the top reagents for this family (including the newly FDA approved Venetoclax), and how they can help you on your research journey.

Figure 1. Interaction of pro-apoptotic Bcl-2 proteins and anti-apoptotic Bcl-2 proteins to modulate apoptosis. Adapted from Clin Cancer Res. 2009 Feb 15;15(4):1126-32. PMID: 19228717.​

 

Markers of Bcl-2 family protein detection 

​​

Bcl-2 family target

Marker

Species reactivity

Key features

Bcl-2


Anti-Bcl-2 antibody

[E17] (ab32124)

Human

Anti-Bcl-2 antibody [EPR17509] (ab182858)

Mouse, human

  • Validated for WB, ICC/IF, flow cytometry, IHC-P 

Bcl-XL

Anti-Bcl-XL antibody [E18] (ab32370)

Mouse, rat, human

  •  Recognizes Bcl-xl, Bcl-xS and Bcl x (beta) but does not cross-react with other Bcl-2 members
  • Validated for WB, IP, IHC and IF/ICC

Mcl-1

Anti-MCL1 antibody [Y37] (ab32087)

Mouse, rat, human

  • Validated for ICC/IF, WB, IHC-P, flow cytometry, IP
  • Does not cross-react with other Bcl-2 members

Bax

Anti-Bax antibody [E63] (ab32503)

Mouse, rat, human, Chinese hamster

Bak

Anti-Bak antibody [Y164] (ab32371)

Human

Anti-Bak antibody [AT8B4] (ab104124)

Mouse, human

  • Validated for WB, ELISA, ICC/IF  

Bad

Anti-Bad antibody [Y208] (ab32445)

Mouse, rat, dog, human

  • Validated for flow cytometry, ICC/IF, IHC-P, IP, WB

Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)

Mouse, rat, human

Bim

Anti-Bim antibody [Y36] (ab32158)

Mouse, rat, human

  • Validated in flow cytometry, ICC/IF, IHC-FoFr, IHC-P, IP, WB



Inhibition and activation of Bcl-2 family proteins 

​​

Bcl-2 family target

Modifier

Activity

Key features

Bcl-2

Venetoclax ABT -199 (ab217298)

Inhibitor

  • Selective Bcl-2 inhibitor
  • BH3-mimetic
  • FDA-approved

Bcl-xl

Z36 (ab141757)

Inhibitor

  • Novel Bcl-xl inhibitor
  • Inhibits cell proliferation and induces autophagy but not apoptosis

Mcl-1

AT 101 (ab145624)

Inhibitor

  • BH3-mimetic
  • Pro-apoptotic agent 

Bcl-2, Bcl-xl and Bcl- w

ABT-737 (ab141336) 

Inhibitor         

  • BH3 mimetic: binds Bcl-2 and Bcl-xl with high affinity
  • Shows antitumor effects in vivo

Pan

Gambogic acid (ab145183)

Inhibitor

  • Apoptotic agent
  • Decreases Bcl-2 and increases Bax expression by competitive inhibition

Bax

BAM7 (ab218116)

Activator

  • Selective Bax activator
  • Targets proapoptotic bax at its trigger site
  • Induces apoptosis in vitro

For more Bcl-2 related products please see here 

Sign up