All tags cancer Choose the most sensitive metastasis antibodies

Choose the most sensitive metastasis antibodies

We compare some of our metastasis antibodies for IHC with the leading alternatives from our competitors. 

Results summary

  • Our N-cadherin, E-cadherin, MMP-2, and collagen I antibodies tested here are all extremely sensitive even at low concentrations
  • Our antibodies consistently show a specific signal with very low background
  • Our E-cadherin (ab40772) and MMP-2 (ab86607) antibodies were shown to perform much better than comparable antibodies from leading competitors 

The IHC images below were made using sections of formalin-fixed paraffin embedded tissue samples. These sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). All primary antibodies were applied for 15 minutes at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The sections were then counterstained with hematoxylin. 

N-Cadherin

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AB
AntibodyRabMAb® N-cadherin monoclonal antibody (ab76011)Competitor N-cadherin rabbit monoclonal antibody
Dilution1µg/mL1µg/mL
Staining description Specific staining with a strong signal. Low background.Specific staining with a weaker signal. Low background.
VerdictExcellentVery good

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E-Cadherin


AB
Antibody

RabMAb® E-cadherin 
monoclonal antibody (ab40772)

Competitor E-cadherin rabbit monoclonal antibody
Dilution1/1,0001/200
Staining description Specific staining with a strong signal. Low background.Specific staining with a weak signal. Low background.
VerdictExcellentPoor

AB
Antibody

Dilution

Staining description 

Verdict



MMP-2


AB
AntibodyMMP-2 mouse monoclonal antibody (ab86607)Competitor MMP-2 mouse monoclonal antibody
Dilution1µg/mL 1µg/mL
Staining description Specific staining with a strong signal. Low background.Specific staining with a weak signal. Low background.
VerdictExcellentPoor


Collagen I


AB
AntibodyCollagen I rabbit polyclonal antibody (ab34710)Competitor collagen I rabbit polyclonal antibody 
Dilution5 µg/mL5 µg/mL
Staining description Specific staining with a strong signal. Low background.Specific staining with a strong signal. Low background.
VerdictExcellentExcellent
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