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Study every aspect of vimentin with our comprehensive range of research tools.
Updated December 15, 2020
Vimentin is a type III intermediate filament protein that anchors and supports organelles within the cytosol of mesenchymal cells. Expression of vimentin is upregulated during epithelial to mesenchymal transition (EMT), a process that occurs during neural development, wound healing, and cancer metastasis. Due to its diverse role within the cell, vimentin can be studied in many contexts ranging from cancer to cytoskeletal dynamics.
Contents
Whatever experimental approach you take, there’s a vimentin antibody to get you excellent results. Pick from unconjugated, conjugated, phospho-specific, or BSA and azide-free. Browse all vimentin antibodies.
Choose from an array of highly validated and cited vimentin antibodies.
Antibody | Host species and clonality | Applications | Species reactivity |
Anti-Vimentin antibody [EPR3776] (ab92547) | Recombinant rabbit monoclonal | WB, Flow Cyt, ICC/IF, IHC-P, IHC-Fr | Human, Mouse, Rat, Rhesus |
Anti-Vimentin antibody [RV202] (ab8978) | Mouse monoclonal | WB, IP, Flow Cyt, ICC/IF, IHC-Fr, IHC-P | Various |
Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) | Recombinant rabbit monoclonal | WB | Human, Mouse, Rat |
Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) | Rabbit monoclonal | WB, IP | Human, Mouse, Rat |
Browse all unconjugated vimentin antibodies
Pair your vimentin primary antibody with the most appropriate secondary. View your guide to secondary antibody selection.
Save time on your labeling protocol with directly conjugated vimentin antibodies. Ideal for multicolor IHC, ICC and flow cytometry experiments. Available conjugated to fluorescent proteins, biotin, HRP and Alexa Fluor® dyes.
Antibody | Host species and clonality | Applications | Species reactivity |
Anti-Vimentin antibody [EPR3776] (Alexa Fluor® 488) (ab185030) | Recombinant rabbit monoclonal | Flow Cyt, ICC/IF | Human, Mouse, Rat |
Anti-Vimentin antibody [EPR3776] (Alexa Fluor® 647) (ab194719) | Recombinant rabbit monoclonal | Flow Cyt, ICC/IF | Mouse |
Anti-Vimentin antibody [EPR3776] - (Alexa Fluor® 594) (ab154207) | Recombinant rabbit monoclonal | Flow Cyt, ICC/IF | Human, Mouse |
Vimentin assembly and function is regulated by phosphorylation1. Detect phosphorylation with our range of antibodies against post-translationally-modified vimentin.
Antibody | Host species and clonality | Species reactivity | Application |
Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) | Recombinant rabbit monoclonal | Mouse, rat, human | ICC/IF, WB |
Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673) | Recombinant rabbit monoclonal | Human | WB, dot blot, ICC/IF, IP, Flow Cyt |
View all phospho vimentin antibodies
Protect the phosphorylation state of your samples with Protease and Phosphatase Inhibitor Cocktail (EDTA-free) (ab201120).
Measure vimentin in human samples with vimentin kits and assays.
Product name | Species reactivity | Sample type |
Human Vimentin Profiling ELISA Kit (ab173190) | Human | Adherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate |
Human Vimentin SimpleStep ELISA® kit (ab246526) | Human | Cell culture extracts |
Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail (ab157392) | Human | Cell lysate |
Product name | Description |
Recombinant human TGF beta 1 protein (ab50036) | EMT inducer2,3,4 |
EGF | EMT inducer4 |
HGF | EMT inducer5 |
BMP-7 The growth factor domain of mouse BMP-7 shares 98% and 100% amino acid sequence identity with human and rat BMP-7, respectively. | EMT inhibitor6,2 |
IL-6 | EMT inducer7 |
Withaferin A from Withania somnifera (ab120644) | Binds to and downregulates vimentin in vascular endothelial cells8 |
Salinomycin sodium salt (ab146178) | Induces EMT and upregulates vimentin expression9 |
SB431542 (ab120163) | EMT inhibitor10 |
Okadaic acid (ab120375) | PP1/PP2A inhibitor that disassembles vimentin intermediate filaments (VIFs) via hyperphosphorylation11,12 |
Calyculin A (ab141784) | PP1/PP2A inhibitor similar to okadaic acid that disassembles VIFs into tetrameric oligomers via hyperphosphorylation13,14 |
(-)-Epigallocatechin gallate (ab120716) | Inhibitor of EMT process via Nrf-2 pathway. Binds vimentin (kd = 3.3 nM) and inhibits vimentin phosphorylation by Cdk2 and PKA15,16,17 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.
11. Whipple, R. A. et al. Vimentin Filaments Support Extension of Tubulin-Based Microtentacles in Detached Breast Tumor Cells. Cancer Res. 68, (2008).
12. Yatsunami, J. et al. Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid. Biochem. Biophys. Res. Commun. 177, 1165–70 (1991).
13. Eriksson, J. E. et al. Specific in vivo phosphorylation sites determine the assembly dynamics of vimentin intermediate filaments. J. Cell Sci. 117, (2004).
14. Tzivion, G., Luo, Z. J. & Avruch, J. Calyculin A-induced vimentin phosphorylation sequesters 14-3-3 and displaces other 14-3-3 partners in vivo. J. Biol. Chem. 275, 29772–8 (2000).
15. Hazgui, S. et al. Epigallocatechin-3-gallate (EGCG) inhibits the migratory behavior of tumor bronchial epithelial cells. Respir. Res. 9, 33 (2008).
16. Kanlaya, R. et al. Protective effect of epigallocatechin-3-gallate (EGCG) via Nrf2 pathway against oxalate-induced epithelial mesenchymal transition (EMT) of renal tubular cells. Sci. Rep. 6, 30233 (2016).
17. Ermakova, S. et al. The intermediate filament protein vimentin is a new target for epigallocatechin gallate. J. Biol. Chem. 280, 16882–90 (2005).