All tags cancer The vimentin toolbox

The vimentin toolbox

Study vimentin from all angles with this comprehensive range of research tools. 

​Vimentin is a type III intermediate filament protein that anchors and supports organelles within the cytosol of mesenchymal cells. Expression of vimentin is upregulated during epithelial to mesenchymal transition (EMT), a process that occurs during neural development, wound healing, and cancer metastasis. Due to its diverse role within the cell, vimentin can be studied in many contexts ranging from cancer to cytoskeletal dynamics.

Click on the links below to browse research tools for vimentin.

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Primary antibodies

Whatever your experiment, there’s a  vimentin antibody to get you excellent results. Pick from unconjugated, conjugated, phospho-specific, or BSA and azide-free. Browse all vimentin antibodies. ​

Unconjugated vimentin antibodies

Choose from an array of highly validated and cited vimentin antibodies. If you’re working on mouse tissue and looking for the specificity and consistency of a monoclonal, why not minimize background with an anti-vimentin recombinant rabbit monoclonal antibody (ab92547)? Currently available in a trial size volume. View the datasheet.

AntibodyHost species and clonalitySpecies reactivityApplication
Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab92547)Rabbit monoclonalMouse, rat, human, rhesus monkeyFlow Cyt, ICC/IF, IHC-Fr, IHC-P, WB
Mouse monoclonal [V9] to Vimentin - Cytoskeleton Marker (ab8069)Mouse monoclonalRat, horse, chicken, cow, cat, dog, human, pigFlow Cyt, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
Anti-Vimentin antibody (ab24525)Chicken polyclonalMouse, rat, humanICC/IF, IHC-FoFr, IHC-Fr, WB
Anti-Vimentin antibody - Cytoskeleton Marker (ab45939)Rabbit polyclonalMouse, rat, rabbit, horse, cow, human (predicted: sheep, chicken, pig, chimpanzee)Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB

Browse all unconjugated vimentin antibodies

Pair your vimentin primary antibody with the best secondary for your experiment. View your guide to secondary antibody selection.
Need to go BSA and azide free? Anti-vimentin recombinant rabbit monoclonal antibody antibody [EPR3776] (ab193555) is available in a BSA and azide-free format. View the datasheet.

Directly conjugated vimentin antibodies

​Pair your vimentin primary antibody with the most appropriate secondary. View your guide to secondary antibody selection.

Need to go BSA and azide-free? Anti-vimentin recombinant rabbit monoclonal antibody antibody [EPR3776] (ab193555) is available in a BSA and azide-free format. View the datasheet.


Save time on your labeling protocol with directly conjugated vimentin antibodies. Ideal for multicolor IHC, ICC and flow cytometry experiments. Available conjugated to fluorescent proteins, biotin, HRP and Alexa Fluor® dyes. 


AntibodyHost species and clonalitySpecies reactivityApplication
Anti-Vimentin antibody [V9] - Cytoskeleton Marker (Alexa Fluor® 488) (ab195877)Mouse monoclonalRat, horse, chicken, cow, cat, dog, human, pigFlow Cyt, ICC/IF
Anti-Vimentin antibody [V9] - Cytoskeleton Marker (Alexa Fluor® 647) (ab195878)Mouse monoclonalRat, horse, chicken, cow, cat, dog, human, pigFlow Cyt, ICC/IF
Anti-Vimentin antibody [EPR3776] (Alexa Fluor® 568) (ab202504)Rabbit monoclonalMouse, humanICC/IF

Browse all directly conjugated vimentin antibodies


Phospho vimentin antibodies

Vimentin assembly and functions are believed to be regulated by phosphorylation1. Detect phosphorylation with our range of antibodies against post-translationally-modified vimentin.

AntibodyHost species and clonality Species reactivityApplication
Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)Rabbit monoclonalMouse, rat, humanICC/IF, WB
Anti-Vimentin (phospho S55) antibody [4A4] (ab22651)Mouse monoclonalMouse, rat, human, ferretELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB
Anti-Vimentin (phospho S71) antibody [TM71] (ab115189)Rat monoclonalMouse, rat, human (predicted: pig)ICC/IF, IHC-P, WB

View all phospho vimentin antibodies

Protect the phosphorylation state of your samples with Protease and Phosphatase Inhibitor Cocktail (EDTA-free) (ab201120).


Kits and assays

Measure vimentin in human samples with vimentin kits and assays.

Product nameSpecies reactivitySample type
Human Vimentin Profiling ELISA Kit (ab173190)HumanAdherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate
Epithelial-Mesenchymal Transition (Beta Catenin, Vimentin) Western Blot Cocktail (ab157392)HumanCell lysate



Modulate vimentin and the process of EMT with a range of effector proteins.

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Product nameDescription
Recombinant human TGF beta 1 protein (ab50036)EMT inducer2,3,4

EGF
Recombinant human EGF protein (Animal Free) (ab9697)
Recombinant mouse EGF protein (ab72994)
Recombinant rat EGF protein (ab198562)

EMT inducer4

HGF
Recombinant human HGF protein (ab632)
​Recombinant mouse HGF protein (ab123229)

EMT inducer5

BMP-7
Recombinant human BMP7 protein (ab50100)

The growth factor domain of mouse BMP-7 shares 98% and 100% amino acid sequence identity with human and rat BMP-7, respectively.

EMT inhibitor6,2

IL-6
Recombinant human IL6 protein (ab9627)​
​Recombinant mouse IL6 protein (ab198572)
Recombinant rat IL6 protein (ab9771)

EMT inducer7
Withaferin A from Withania somnifera (ab120644)Binds to and downregulates vimentin in vascular endothelial cells8
Salinomycin sodium salt (ab146178)Induces EMT and upregulates vimentin expression9
SB431542 (ab120163)EMT inhibitor10
Okadaic acid (ab120375)PP1/PP2A inhibitor that disassembles vimentin intermediate filaments (VIFs) via hyperphosphorylation11,12
Calyculin A (ab141784)PP1/PP2A inhibitor similar to okadaic acid that disassembles VIFs into tetrameric oligomers via hyperphosphorylation13,14
(-)-Epigallocatechin gallate (ab120716)Inhibitor of EMT process via Nrf-2 pathway. Binds vimentin (kd = 3.3 nM) and inhibits vimentin phosphorylation by Cdk2 and PKA15,16,17

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.


References

1. Cogli, L., Progida, C., Bramato, R. & Bucci, C. Vimentin phosphorylation and assembly are regulated by the small GTPase Rab7a. Biochim. Biophys. Acta - Mol. Cell Res. 1833, 1283–1293 (2013).

2. Zeisberg, M. et al. BMP-7 counteracts TGF-β1–induced epithelial-to-mesenchymal transition and reverses chronic renal injury. Nat. Med. 9, 964–968 (2003).

3. Kalluri, R. & Weinberg, R. A. The basics of epithelial-mesenchymal transition. J. Clin. Invest. 119, 1420–1428 (2009).

4. Grände, M. et al. Transforming growth factor-beta and epidermal growth factor synergistically stimulate epithelial to mesenchymal transition (EMT) through a MEK-dependent mechanism in primary cultured pig thyrocytes. J. Cell Sci. 115, 4227–4236 (2002).

5. Hu, H.-J., Lin, X.-L., Liu, M.-H., Fan, X.-J. & Zou, W.-W. Curcumin mediates reversion of HGF-induced epithelial-mesenchymal transition via inhibition of c-Met expression in DU145 cells. Oncol. Lett. 11, 1499–1505 (2016).

6. Wang, Y. et al. Effect of bone morphogenic protein-7 on the expression of epithelial-mesenchymal transition markers in silicosis model. Exp. Mol. Pathol. 98, 393–402 (2015).

7. Sullivan, N. et al. Interleukin-6 induces an epithelial–mesenchymal transition phenotype in human breast cancer cells. Oncogene 28, 2940–2947 (2009).

8. Bargagna-Mohan, P. et al. The tumor inhibitor and antiangiogenic agent withaferin A targets the intermediate filament protein vimentin. Chem. Biol. 14, 623–34 (2007).

9. Koeck, S. et al. The impact of metformin and salinomycin on transforming growth factor β-induced epithelial-to-mesenchymal transition in non-small cell lung cancer cell lines. Oncol. Lett. 11, 2946–2952 (2016).

10. Zhang, X. et al. Smad2/3 Upregulates the Expression of Vimentin and Affects Its Distribution in DBP-Exposed Sertoli Cells. PPAR Res. 2015, 1–11 (2015).

11. Whipple, R. A. et al. Vimentin Filaments Support Extension of Tubulin-Based Microtentacles in Detached Breast Tumor Cells. Cancer Res. 68, (2008).

12. Yatsunami, J. et al. Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid. Biochem. Biophys. Res. Commun. 177, 1165–70 (1991).

13. Eriksson, J. E. et al. Specific in vivo phosphorylation sites determine the assembly dynamics of vimentin intermediate filaments. J. Cell Sci. 117, (2004).

14. Tzivion, G., Luo, Z. J. & Avruch, J. Calyculin A-induced vimentin phosphorylation sequesters 14-3-3 and displaces other 14-3-3 partners in vivo. J. Biol. Chem. 275, 29772–8 (2000).

15. Hazgui, S. et al. Epigallocatechin-3-gallate (EGCG) inhibits the migratory behavior of tumor bronchial epithelial cells. Respir. Res. 9, 33 (2008).

16. Kanlaya, R. et al. Protective effect of epigallocatechin-3-gallate (EGCG) via Nrf2 pathway against oxalate-induced epithelial mesenchymal transition (EMT) of renal tubular cells. Sci. Rep. 6, 30233 (2016).

17. Ermakova, S. et al. The intermediate filament protein vimentin is a new target for epigallocatechin gallate. J. Biol. Chem. 280, 16882–90 (2005).


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