Overview

  • Product name
    Anti-Candida albicans antibody
    See all Candida albicans primary antibodies
  • Description
    Rabbit polyclonal to Candida albicans
  • Specificity
    ab53891 has not been absorbed and cross reacts with other yeasts. It reacts with numerous proteins in a soluble C. albicans extract in immunoelectrophoresis.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae, Candida albicans
    Does not react with: Human
  • Immunogen

    Candida albicans, type A

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Candida albicans is the most frequently isolated fungal pathogen of humans, affecting immunocompromised patients ranging from premature infants to AIDS sufferers. Systemic infections have an attributed mortality of 30-50%. C. albicans is a diploid organism which has eight sets of homologous chromosomes. It has a genome of approximately 16 Mb (haploid), about 30% greater than S. cerevisiae (baker’s yeast).
  • Alternative names
    • C albicans antibody
    • Thrush antibody

Images

  • ab53891 staining Candida albicans (sc5314) by Flow Cytometry. The sample was incubated with the primary antibody (1/500 in DPBS/3% fetal calf serum/10% goat serum) for 1 hour at 4°C. An Allophycocyanin-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Gating Strategy: FSC/SSC - histogram of single stained population.

    See Abreview

References

This product has been referenced in:
  • Navarathna DH  et al. CD47 Promotes Protective Innate and Adaptive Immunity in a Mouse Model of Disseminated Candidiasis. PLoS One 10:e0128220 (2015). Read more (PubMed: 26010544) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
eBioScience IC Fixation Buffer
Permeabilization
No
Sample
Candida albicans Cell (sc5314)
Specification
sc5314
Gating Strategy
FSC/SSC--> histogram of single stained population
Preparation
Cell harvesting/tissue preparation method: YPD growth medium O/N (single colony), wash 3x DPBS
Sample buffer: DPBS/3% Fetal calf serum
Username

Mrs. Erica Stein

Verified customer

Submitted Jun 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tongue)
Specification
tongue
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Mar 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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