Anti-Candida albicans antibody (ab53891)

Overview

  • Product nameAnti-Candida albicans antibody
    See all Candida albicans primary antibodies
  • Description
    Rabbit polyclonal to Candida albicans
  • Specificityab53891 has not been absorbed and cross reacts with other yeasts. It reacts with numerous proteins in a soluble C. albicans extract in immunoelectrophoresis.
  • Tested applicationsSuitable for: ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae, Candida albicans
    Does not react with: Human
  • Immunogen

    Candida albicans, type A

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceCandida albicans is the most frequently isolated fungal pathogen of humans, affecting immunocompromised patients ranging from premature infants to AIDS sufferers. Systemic infections have an attributed mortality of 30-50%. C. albicans is a diploid organism which has eight sets of homologous chromosomes. It has a genome of approximately 16 Mb (haploid), about 30% greater than S. cerevisiae (baker’s yeast).
  • Alternative names
    • C albicans antibody
    • Thrush antibody

Anti-Candida albicans antibody images

  • ab53891 staining Candida albicans (sc5314) by Flow Cytometry. The sample was incubated with the primary antibody (1/500 in DPBS/3% fetal calf serum/10% goat serum) for 1 hour at 4°C. An Allophycocyanin-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Gating Strategy: FSC/SSC - histogram of single stained population.

    See Abreview

References for Anti-Candida albicans antibody (ab53891)

ab53891 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation eBioScience IC Fixation Buffer
Permeabilization No
Sample Candida albicans Cell (sc5314)
Specification sc5314
Gating Strategy FSC/SSC--> histogram of single stained population
Preparation Cell harvesting/tissue preparation method: YPD growth medium O/N (single colony), wash 3x DPBS
Sample buffer: DPBS/3% Fetal calf serum
Username

Mrs. Erica Stein

Verified customer

Submitted Jun 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tongue)
Specification tongue
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Mar 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"