Canine alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab157696)


  • Product name
    Canine alpha 1 Antitrypsin ELISA Kit (SERPINA1)
    See all alpha 1 Antitrypsin kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma, Other biological fluids
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    4.48 ng/ml
  • Range
    12.5 ng/ml - 800 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Dog
    Does not react with: Horse
  • Product overview

    Abcam’s Alpha 1 Antitrypsin (SERPINA1) Dog ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Alpha 1 Antitrypsin in biological samples of Dog.


    In this assay the Alpha 1 Antitrypsin present in samples reacts with the anti- Alpha 1 Antitrypsin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- Alpha 1 Antitrypsin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Alpha 1 Antitrypsin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1 Antitrypsin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1 Antitrypsin in the test sample. The quantity of Alpha 1 Antitrypsin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-conjugated anti-dog alpha 1 Antitrypsin antibody 1 x 150µl
    20X Wash Buffer Concentrate 1 x 50ml
    5X Diluent Concentrate 1 x 50ml
    Chromogen Substrate Solution 1 x 12ml
    Dog alpha 1 Antitrypsin Calibrator (Lyophilized) 1 vial
    Dog alpha 1 Antitrypsin ELISA Microplate 1 unit
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificity
    Ubiquitous. Expressed in leukocytes and plasma.
  • Involvement in disease
    Alpha-1-antitrypsin deficiency
  • Sequence similarities
    Belongs to the serpin family.
  • Domain
    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localization
    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Alternative names
    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all


Our Abpromise guarantee covers the use of ab157696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • representative standard curve using ab157696 alpha 1 Antitrypsin Dog ELISA Kit



ab157696 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The concentration of AAT in ferret plasma

Excellent Excellent 5/5 (Ease of Use)
The protocol is as the instruction of the Elisa kit. But the concentration we tested is at the level of ng/ml. While other species is at the level of mg/ml.

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Verified customer

Submitted Jul 06 2016


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