Overview

  • Product name
    Canine IFN-gamma ELISA Kit (IFNG)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.1 ng/ml
  • Range
    0.1 ng/ml - 25 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 77.11 69% - 85%
    Plasma 81.29 68% - 95%
    Cell culture media = 73.83 68% - 99%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Dog
    Does not react with: Mouse, Rat, Horse, Cow, Cat, Human, Pig
  • Product overview

    Abcam’s IFN gamma canine ELISA Kit (ab193684) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine IFN gamma in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant. Heparin is not recommended for use in this assay) and cell culture supernatants.

    This assay employs an antibody specific for canine IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IFN gamma present in the samples. The wells are washed and biotinylated anti-canine IFN gamma antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IFN gamma bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated Canine IFN-gamma Detection Antibody 2 vials
    Canine IFN-gamma Standards 2 vials
    Pre-coated Canine IFN-gamma Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Cellular localization
    Secreted
  • Alternative names
    • IFG
    • IFI
    • IFN-gamma
    • IFNG
    • Immune interferon
    • Interferon gamma
    see all

Applications

Our Abpromise guarantee covers the use of ab193684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193684 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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