- Datasheet
- Specific References
- Protocols
Overview
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Product nameCanine IgG ELISA Kit
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Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Serum 3 2.7% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample typeSerum
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Assay typeSandwich (quantitative)
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Sensitivity100 pg/ml
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Range0.31 ng/ml - 20 ng/ml
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Recovery
Sample specific recovery Sample type Average % Range Cell culture media 101 98% - 106% Fetal Bovine Serum 117 112% - 128% 1X Wash Buffer 112 109% - 118% -
Assay time1h 30m
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Assay durationOne step assay
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Species reactivityReacts with: Dog
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Product overview
IgG canine in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of canine IgG in serum samples.
The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Notes
There are six classes of immunoglobulins in canine: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin in serum and is equally distributed in blood and tissue. In canine, the IgG class is further divided into four subclasses: IgG1, IgG2, IgG3, and IgG4. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y ike structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.
IgG is involved in response to a foreign antigen and the presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, can bind to many pathogens and also plays an important role in antibody dependent cell-mediated cytotoxicity. -
Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Canine IgG Capture Antibody 1 x 600µl 10X Canine IgG Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CP 1 x 6ml Canine IgG Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml -
RelevanceImmunoglobulin G (IgG), is one of the most abundant proteins in serum. IgG is important for defence against microorganisms and the molecules are produced by B-lymphocytes as a part of the adaptive immune response. The IgG molecule has two separate functions: to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Applications
Our Abpromise guarantee covers the use of ab193768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Example of canine IgG standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of canine serum IgG level in Sample Diluent NS.Background-subtracted data values (mean +/- SD, n = 3) are graphed.
Protocols
References
ab193768 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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