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|Sample type||Average %||Range|
|Serum||110.2||102% - 118%|
|Plasma||103.2||84% - 122%|
|Cell culture media||128.2||122% - 132%|
Abcam’s Interleukin-8 (IL-8) Canine ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of dog IL-8 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for dog IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-dog IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|Detection Antibody IL-8 (biotinylated anti-Canine IL-8)||2 vials|
|IL-8 Microplate (12 strips x 8 wells) coated with anti canine IL-8||1 unit|
|IL-8 Standard canine (recombinant)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab155465 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"