Anti-Cannabinoid Receptor I antibody (ab107817)

Overview

  • Product nameAnti-Cannabinoid Receptor I antibody
    See all Cannabinoid Receptor I primary antibodies
  • Description
    Rabbit polyclonal to Cannabinoid Receptor I
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Horse, Cow, Dog, Human, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Cannabinoid Receptor I.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Forebrain; Rat Brain; Rat Forebrain. It also gave a positive signal in mouse brain FFPE tissue sections with Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab107817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-Cannabinoid Receptor I antibody images

  • IHC image of ab107817 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107817, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-Cannabinoid Receptor I antibody (ab107817) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Forebrain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Rat Forebrain Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Cannabinoid Receptor I antibody (ab107817)

ab107817 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"