Anti-Cannabinoid Receptor I antibody (ab23703)

Overview

  • Product nameAnti-Cannabinoid Receptor I antibody
    See all Cannabinoid Receptor I primary antibodies
  • Description
    Rabbit polyclonal to Cannabinoid Receptor I
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Cow, Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    MSVSTDTSAE AL

    , corresponding to C terminal amino acids 461-472 of Human Cannabinoid Receptor I(Peptide available as ab50542.)

  • Positive control
    • Human cerebellum or murine brain sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab23703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).Can be blocked with Cannabinoid Receptor I peptide (ab50542).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/300. Detection with an ABC system is recommended.
ICC/IF 1/50.

Target

Anti-Cannabinoid Receptor I antibody images

  • Anti-Cannabinoid Receptor I antibody (ab23703) + Human cerebellum at 30 µg

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunoperoxidase staining of CA region of rat hippocampus with the Cannabinoid Receptor I antibody (ab23703, 4ug/ml). the majority of the protein is localized in the pre-synaptic axons, shown as brown particulaes. A few neurons also show cytoplasm staining.
  • ab23703 staining Cannabinoid Receptor I in rat brain tissue by Immunohistochemistry (Frozen sections). After fixation in 4% PFA in PBS (pH 7.4), brains were cryoprotected in 30% sucrose in PBS at +4°C, frozen, and thereafter kept at -80°C until used. For the immunostaining, brains were cryosectioned in 30 µm slices, collected in PBS (pH 7.4), and processed in a free-floating system. Slices were first incubated in the blocking solution (BS) containing 2% bovine serum albumin, 2% goat serum, and 0.1% Triton X-100 in PBS (pH 7.4) for 1 hour at room temperature, and thereafter with the primary antibody for 24–48 hours at +4°C in BS at a 1/1000 dilution. After washing in PBS, slices were incubated with the biotin-conjugated secondary antibody (1/4000) in BS, rinsed with PBS, and incubated with the avidin-peroxidase conjugate in BS for 1 hour at room temperature. The staining was detected using 3,3-diaminobenzidinetetrahydrochloride as a chromogen.
  • Immunofluorescence analysis of mouse cultured hippocampal neurons, staining Cannabinoid Receptor I with ab23703.

    Cells were fixed with paraformaldehyde, permeabilized with 0.05% Saponin and blocked with 10% fetal calf serum for 2 hous at 4°C. Samples were incubated with primary antibody (1/200 in 0.05% Saponin + fetal calf serum) for 12 hours at 23°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

References for Anti-Cannabinoid Receptor I antibody (ab23703)

This product has been referenced in:
  • von Rüden EL  et al. Analysis in conditional cannabinoid 1 receptor-knockout mice reveals neuronal subpopulation-specific effects on epileptogenesis in the kindling paradigm. Neurobiol Dis 73:334-47 (2015). Mouse . Read more (PubMed: 25123336) »
  • Meng XD  et al. Astrocytic expression of cannabinoid type 1 receptor in rat and human sclerotic hippocampi. Int J Clin Exp Pathol 7:2825-37 (2014). IHC . Read more (PubMed: 25031702) »

See all 14 Publications for this product

Product Wall

Application Flow Cytometry
Sample Mouse Cell (Spleen cells)
Permeabilization No
Gating Strategy live cells
Specification Spleen cells
Preparation Cell harvesting/tissue preparation method: cell culture (RPMI)
Sample buffer: PBS 1x
Fixation Fresh cells
Username

Ms. Coral González García

Verified customer

Submitted Aug 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Sample Cat Tissue sections (Felis catus brain parietal cortex)
Specification Felis catus brain parietal cortex
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 05 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative 10% buffered formalin
Username

Abcam user community

Verified customer

Submitted Oct 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Sample Rat Tissue lysate - whole (bladder)
Specification bladder
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. EUN-YOUNG PARK

Verified customer

Submitted Jul 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (mouse brain - post nuclear supernatant)
Loading amount 20 µg
Specification mouse brain - post nuclear supernatant
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T)
Loading amount 20 µg
Specification HepG2, 293T
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted Feb 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cultured hippocampal neurons)
Specification cultured hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0,05% (w/v) saponin in PBS
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse brain sections)
Specification mouse brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - Triton during block and staining
Blocking step PBS, 10%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2013

Thank you for contacting Abcam.

Earlier we discussed your use of ab23703 for IHC-P, specifically the lack of a positive signal with a 72oC Citrate buffer antigen retrieval. We discussed some troubleshooting tips, specifically attempting a 30 m...

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I am sorry to hear you have been experiencing problems with this antibody. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"