Anti-Cannabinoid Receptor I antibody (ab3558)
Key features and details
- Rabbit polyclonal to Cannabinoid Receptor I
- Suitable for: IP, ICC/IF, IHC-Fr, IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Rat, Rabbit, Human, Ferret
- Isotype: IgG
Overview
-
Product name
Anti-Cannabinoid Receptor I antibody
See all Cannabinoid Receptor I primary antibodies -
Description
Rabbit polyclonal to Cannabinoid Receptor I -
Host species
Rabbit -
Specificity
Detects CB1 from Human and Rat tissues as well as transfected Rat CB1. -
Tested applications
Suitable for: IP, ICC/IF, IHC-Fr, IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human, Ferret
Predicted to work with: Cat, Chimpanzee, Rhesus monkey -
Immunogen
Recombinant fragment within Human Cannabinoid Receptor I aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA, 49% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration. PubMed: 22387618
|
|
ICC/IF |
1/1000.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration. PubMed: 20206138
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
By Western blot, this antibody detects an ~60 kDa protein representing CB1 from rat brain homogenate. |
Notes |
---|
IP
Use at an assay dependent concentration. PubMed: 22387618 |
ICC/IF
1/1000. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 20206138 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa. By Western blot, this antibody detects an ~60 kDa protein representing CB1 from rat brain homogenate. |
Target
-
Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: O02777 Cat
- SwissProt: Q5IS73 Chimpanzee
- SwissProt: P21554 Human
- SwissProt: P47746 Mouse
see all -
Alternative names
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab3558 has been referenced in 5 publications.
- Liu AN et al. The Effects of HP0044 and HP1275 Knockout Mutations on the Structure and Function of Lipopolysaccharide in Helicobacter pylori Strain 26695. Biomedicines 10:N/A (2022). PubMed: 35052824
- Wang Y et al. Low-Dose Delta-9-Tetrahydrocannabinol as Beneficial Treatment for Aged APP/PS1 Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35269905
- Kleyer J et al. Cannabinoid receptor trafficking in peripheral cells is dynamically regulated by a binary biochemical switch. Biochem Pharmacol 83:1393-412 (2012). Flow Cyt, IP ; Human . PubMed: 22387618
- Shen L et al. The role of peripheral cannabinoid receptors type 1 in rats with visceral hypersensitivity induced by chronic restraint stress. J Neurogastroenterol Motil 16:281-90 (2010). WB ; Rat . PubMed: 20680167
- Leonti M et al. Falcarinol is a covalent cannabinoid CB1 receptor antagonist and induces pro-allergic effects in skin. Biochem Pharmacol 79:1815-26 (2010). WB, Flow Cyt ; Human . PubMed: 20206138