Anti-Cannabinoid Receptor I antibody (ab3558)

Overview

  • Product nameAnti-Cannabinoid Receptor I antibody
    See all Cannabinoid Receptor I primary antibodies
  • Description
    Rabbit polyclonal to Cannabinoid Receptor I
  • SpecificityDetects CB1 from Human and Rat tissues as well as transfected Rat CB1.
  • Tested applicationsSuitable for: ICC, WB, IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Ferret
    Predicted to work with: Cat, Chimpanzee, Rhesus monkey
  • Immunogen

    Fusion protein corresponding to Human Cannabinoid Receptor I aa 1-99 (N terminal).

Properties

Applications

Our Abpromise guarantee covers the use of ab3558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/1000. Immunocytochemical staining of CB1 in AtT20 cells transfected with the rat CB1 gene yields a pattern consistent with plasma membrane staining.
WB 1/250. By Western blot, this antibody detects an ~60 kDa protein representing CB1 from rat brain homogenate.
IP Use at an assay dependent concentration. PubMed: 22387618
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20206138

Target

Anti-Cannabinoid Receptor I antibody images

  • Quantification of CB1 and CB2 receptor surface expression in primary Human and HaCaT keratinocytes. (A) FL-1 shows autofluorescence (black population), non-specific binding of secondary antibody (dark grey population) and specific immunofluorescence obtained with CB1 (ab3558) and CB2 (ab3561) receptor antibodies, respectively (light grey population). Histogram shows representative measurement (5000 cells counted). (B) Differences in CB receptor expression between primary and HaCaT keratinocytes, showing marked increased expression of CB2 in primary keratinocytes (geo mean corrected for non-specific binding of secondary Ab). Data show mean values ±SEM obtained with two human primary keratinocyte samples and HaCaT cells, each measured three times.

  • ab3558 at a 1:1000 dilution staining Rat CB1 in transfected AtT20 cells by immunocytochemistry.

References for Anti-Cannabinoid Receptor I antibody (ab3558)

This product has been referenced in:
  • Kleyer J  et al. Cannabinoid receptor trafficking in peripheral cells is dynamically regulated by a binary biochemical switch. Biochem Pharmacol 83:1393-412 (2012). Flow Cyt, IP ; Human . Read more (PubMed: 22387618) »
  • Shen L  et al. The role of peripheral cannabinoid receptors type 1 in rats with visceral hypersensitivity induced by chronic restraint stress. J Neurogastroenterol Motil 16:281-90 (2010). WB ; Rat . Read more (PubMed: 20680167) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

We have following CB1 and CB2 available. These are testes in flow cytometry and are fully guaranteed.

ab3558; http://www.abcam.com/Cannabinoid-Receptor-I-antibody-ab3558.html

ab3561; http://www.ab...

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Thank you for your inquiry.

The best way to find isotype controls for an antibody is to start at our homepage www.abcam.com. Go to the yellow bar at the top of the page and move the mouse to “Products,” then click on “Iso...

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Thank you for your enqiury. According to our most recent internal testing data, by Western blot, ab3558 Cannabinoid Receptor I antibody shows detection of an ~ 52 kDa band in rat brain homogenate. After further research on anti-Cannabinoid Rece...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (adult brain)
Specification adult brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 16 2007

Thank you for your message which has been forwarded to me on behalf of Tanya while she is away. It is with regret that we are not able to guarantee this product if it has not been stored as indicated on the datasheet. However, the results indicate ...

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Thank you for your email. I'm sorry that this antibody is not working out under your conditions and I have instructed our accounting department to issue a refund for your order for ab3558. If you have any questions regarding the refund, please contact ...

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Thank you for your email and patience. I do suggest decreasing the concentration of the primary as well as the incubation period as the bands you are seeing may be non-specific. If you are using the same secondary with the Pten primary and not getting...

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Thank you for your email. Yes, this has been tested and characterized in Western blotting as well as Immunocytochemistry. In Western blotting, this antibody detects an ~60 kDa protein representing CB1 from rat brain homogenate. The bands that you are s...

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Thank you for your email. I recall that you were previously unable to obtain any bands with this antibody. I would like to investigate this present issue further but do need details regarding your protocol in order to do that. If you could answer the q...

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There have been no previously reported problems with this particulat lot. It is a possibility that the antibody became defective during shipment. We will send you a replacement vial free of charge, please let me know how it works out for you.

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"