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Fusion protein corresponding to Rat Cannabinoid Receptor I aa 1-77 (N terminal).
Our Abpromise guarantee covers the use of ab3559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. See Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat hippocampus tissue sections labeling Cannabinoid Receptor I with ab3559.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of transverse spinal cord E6 chick embryo tissue sections labeling Cannabinoid Receptor I with ab3559.
ab3559 staining the Cannabinoid Receptor 1 in Human synovial fibroblasts by Flow Cytometry. Cells were fixed with 1.5% formaldehyde, pelleted and permeabilized with 100% ice cold methanol for at least 10 minutes. Gating was done on synovial fibroblasts. The sample was incubated with the primary antibody, dilution 1/100 (1% BSA in PBS), for 15 minutes. An Abcam PE-conjugated donkey polyclonal to rabbit IgG ( ab7007), dilution 1/200, was used as secondary antibody. The red peak shows staining with ab3559.
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