The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 46, 53 kDa (predicted molecular weight: 53 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.
Belongs to the G-protein coupled receptor 1 family.
Flow cytometry analysis of Cannabinoid Receptor I (phospho S316) expression in 2% paraformaldehyde-fixed SH-SY5Y cells using ab186428 at 1/200 dilution (red) or a rabbit IgG isotype control (green), SH-SY5Y cells preincubated with Cannabinoid Receptor I (phospho S316) peptide (blue) or non-phosopho Cannabinoid Receptor I (phospho S316) peptide (orange). FITC-conjugated goat anti-rabbit IgG (1/150 dilution) was used as the secondary antibody.