Anti-Cannabinoid Receptor II antibody (ab3561)


  • Product nameAnti-Cannabinoid Receptor II antibody
    See all Cannabinoid Receptor II primary antibodies
  • Description
    Rabbit polyclonal to Cannabinoid Receptor II
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICC, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chinese Hamster
  • Immunogen

    Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal).

  • Positive control
    • AtT20 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesCannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab3561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
WB 1/50 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P 1/10 - 1/100.
ICC 1/500.
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration. PubMed: 18178718


  • FunctionHeterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • Tissue specificityPreferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • Cellular localizationCell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all

Anti-Cannabinoid Receptor II antibody images

  • ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : Rat colon cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • ab3561 staining Cannabinoid Receptor II in murine BV2 cells by Immunocytochemistry/ Immunofluorescence. Cells were stimulated with 10 uM ADP for 15 minutes at 37°C, 5%CO2 then washed 3X with PBS and fixed with 2% formaldehyde for 15 minutes at room temperature. A blocking step was performed for 1 hour at room temperature with 5% FBS/PBS/0.01% Triton-X-100. ab3561 used at a 1/500 dilution for 1 hour at room temperature then washed 3X PBS. The secondary was an Alexa Fluor 488 conjugated antibody, used at a 1/250 for 1 hour at room temperature, washed 3X PBS then mounted in Vectashield containing DAPI.
  • Quantification of CB1 and CB2 receptor surface expression in primary Human and HaCaT keratinocytes. (A) FL-1 shows autofluorescence (black population), non-specific binding of secondary antibody (dark grey population) and specific immunofluorescence obtained with CB1 (ab3558) and CB2 (ab3561) receptor antibodies, respectively (light grey population). Histogram shows representative measurement (5000 cells counted). (B) Differences in CB receptor expression between primary and HaCaT keratinocytes, showing marked increased expression of CB2 in primary keratinocytes (geo mean corrected for non-specific binding of secondary Ab). Data show mean values ±SEM obtained with two human primary keratinocyte samples and HaCaT cells, each measured three times.

  • ab3561 at 1/5000 staining Rat spinal cord tissue sections by IHC-P. Following nerve trans-section, the animal was transcardially perfused with PBS followed by 4% formaldehyde (no post fixation) then frozen in OCT and stored at -80°C until required. 20µm sections were cut and stained with the antibody for 18 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.

References for Anti-Cannabinoid Receptor II antibody (ab3561)

This product has been referenced in:
  • Latini L  et al. Cannabinoid CB2 receptor (CB2R) stimulation delays rubrospinal mitochondrial-dependent degeneration and improves functional recovery after spinal cord hemisection by ERK1/2 inactivation. Cell Death Dis 5:e1404 (2014). Read more (PubMed: 25188514) »
  • Zhang HY  et al. Cannabinoid CB2 receptors modulate midbrain dopamine neuronal activity and dopamine-related behavior in mice. Proc Natl Acad Sci U S A 111:E5007-15 (2014). IHC ; Mouse . Read more (PubMed: 25368177) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT29 cell line (colon cancer epithelial cell line))
Permeabilization Yes - Tween 0,25%
Specification HT29 cell line (colon cancer epithelial cell line)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Methanol

Abcam user community

Verified customer

Submitted Oct 30 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (bladder)
Loading amount 100 µg
Specification bladder
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C


Verified customer

Submitted Apr 09 2013

Thank you for that clarification.

I have sent you a FedEx link that you can complete to be able to return the vial of ab3561 from order number *. Once that order is returned to us, we will credit the cost of that product back to you. The cred...

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Thank you for that information.

That order number (*), is for the vial of ab3561 that you just received today, correct? If so, I will go ahead and set up the return and credit for this order.

Do you have the order number for the fir...

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Thank you for your reply.

I am sorry about the frustration you are having with ab3561 in flow cytometry and I would be happy to replace the first vial that you received. Of the two possible replacements you mentioned in you previous email, we...

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Thank you for contacting Abcam.

The antibody is covered under our Abpromise for six months and is guaranteed to work in flow cytometry on a number of samples. If we cannot resolve the issue you are having with the antibody then I would be hap...

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Thank you for contacting us.

The number of slides that could be used with this kit depends on the tissue sections, and the volume of reagents per slide. The volume used should be enough to cover them. We normally use 200ul per slide in house,...

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Thank you very much for your reply and for sending the additional information.

Your credit note ID is ***

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original orde...

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Thank you very much for your call today and for letting us know about the trouble with this antibody.

If you could please provide the following information I will arrange the refund promptly:

1) What kind of mouse samples were used ...

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Thank you for contacting us.

We have following CB1 and CB2 available. These are testes in flow cytometry and are fully guaranteed.


ab3561; http://www.ab...

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